Quantitative analysis by liquid chromatography tandem mass spectrometry of deuterium-labeled and unlabeled vitamin E in biological samples

被引:63
作者
Lauridsen, C [1 ]
Leonard, SW
Griffin, DA
Liebler, DC
McClure, TD
Traber, MG
机构
[1] Danish Inst Agr Sci, Res Ctr Foulum, Dept Anim Nutr & Physiol, Corvallis, OR 97331 USA
[2] Oregon State Univ, Linus Pauling Inst Sci & Med, Corvallis, OR 97331 USA
[3] Oregon State Univ, Dept Chem, Corvallis, OR 97331 USA
[4] Univ Arizona, Dept Pharmacol & Toxicol, Tucson, AZ 85721 USA
[5] Novartis Agr Discovery Inst Inc, San Diego, CA 92121 USA
[6] Univ Calif Davis, Sch Med, Div Pulm & Crit Care, Davis, CA 95817 USA
关键词
APCI; pig; human; alpha-tocopherol; LC-MS/MS; oxidation products;
D O I
10.1006/abio.2000.4913
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for quantification of unlabeled a-tocopherol and the deuterated tocopherols, RRR-alpha -5-(CD3)-tocopherol (d(3)RRR) and all rac-alpha -5,7-(CD3)(2) tocopherol (d(6)all-rac) in plasma by HPLC-tandem mass spectrometry (LC-MS/MS) has been developed. Deuterated and unlabeled alpha -tocopherols were separated by HPLC and were detected by positive ion multiple-reaction monitoring using a triple-quadrupole mass spectrometer equipped with a heated nebulizer-atmospheric pressure chemical ionization interface, following routine extraction of vitamin E from plasma. The accuracy and precision were evaluated by replicate analysis of standards and samples. Human plasma samples, which were obtained at different times after the subject had consumed a capsule containing 1:1 ratio of d(3)RRR and d(6)all rac-alpha -tocopheryl acetates, were analyzed with this method. Plasma deuterated alpha -tocopherols measured by LC-MS/MS followed the same pattern as previously demonstrated by GC-MS measurement, without requiring an extra derivitization step. The detection limit was 10 pmol for each form of alpha -tocopherol injected. (C) 2001 Academic Press.
引用
收藏
页码:89 / 95
页数:7
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