Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae

被引:81
作者
Tzanakaki, G [1 ]
Tsopanomichalou, M [1 ]
Kesanopoulos, K [1 ]
Matzourani, R [1 ]
Sioumala, M [1 ]
Tabaki, A [1 ]
Kremastinou, J [1 ]
机构
[1] Natl Sch Publ Hlth, Natl Meningococcal Reference Lab, Athens, Greece
关键词
diagnosis; Haemophilus influenzae; meningitis; Neisseria meningitidis; PCR; Streptococcus pneumoniae;
D O I
10.1111/j.1469-0691.2005.01109.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Rapid, accurate and inexpensive diagnosis of bacterial meningitis is critical for patient management. This study describes the development and evaluation of a multiplex PCR assay for the detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae type b, which globally account for 90% of cases of bacterial meningitis. The single-tube assay, based on the ctrA, ply and bex targets, respectively, enabled detection of 5-10 pg DNA. When the assay was tested with clinical samples (n = 425), its sensitivity for the three targets was 93.9%, 92.3% and 88%, respectively, while the overall specificity and positive predictive value of the assay was 100%. The negative predictive value was 99.1-99.5%. The methodology permits rapid and accurate detection of the three main pathogens that cause bacterial meningitis.
引用
收藏
页码:386 / 390
页数:5
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