Improving the fermentation medium for Echinocandin B production. Part I: sequential statistical experimental design

被引：18

作者：

Cockshott, AR ^{[1
]}

Sullivan, GR ^{[1
]}

机构：

[1] Eli Lilly & Co, Lilly Res Labs, Lilly Corp Ctr, Indianapolis, IN 46285 USA

来源：

PROCESS BIOCHEMISTRY | 2001年 / 36卷 / 07期

关键词：

medium optimization; statistical design; sequential experimentation; ridge analysis;

D O I：

10.1016/S0032-9592(00)00262-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequential statistical experimental designs (Plackett-Burman, factorial, response surface and ridge analysis) were used to improve the medium for the production of Echinocandin B (ECB) by Aspergillus nidulans. Special attention is given to the ridge analysis, a search and optimization technique which can be applied to second-order models. Fifteen medium ingredients were investigated using a total of 108 shake flask fermentations over five stages of experimentation. The iterative process of thoughtful design and careful data analysis ultimately produced a simple, five component media, which increased titre by 46%. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：647 / 660

页数：14

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