Morphological and phenotypical characterization of human endothelial progenitor cells in an early stage of differentiation

被引：27

作者：

Bellik, L ^{[1
]}

Ledda, F ^{[1
]}

Parenti, A ^{[1
]}

机构：

[1] Univ Florence, Dept Preclin & Clin Pharmacol, I-50139 Florence, Italy

来源：

FEBS LETTERS | 2005年 / 579卷 / 12期

关键词：

peripheral blood mononuclear cell; endothelial progenitor cell; early differentiation;

D O I：

10.1016/j.febslet.2005.04.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The exact phenotype and lineage of endothelial progenitor cells (EPCs) are still a matter of debate and different expansion protocols are used to obtain them. In this study, EPC expansion from peripheral blood mononuclear cells was analyzed within the first week of culture. Both the adherent and suspended cells, of which the latter usually discarded, were considered. We provide, for the first time, a systematic study of EPC phenotype and functional features within the first 3 days of culture. Moreover, within the 2nd day, both cellular fractions displayed a significant increase in endothelial marker expression which correlated with EPC properties. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2731 / 2736

页数：6

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