Molecular confirmation of human immunodeficiency virus (HIV) type 2 in HIV-seropositive subjects in South India

被引：19

作者：

Kannangai, R

Ramalingam, S

Prakash, KJ

Abraham, OC

George, R

Castillo, RC

Schwartz, DH

Jesudason, MV

Sridharan, G ^{[1
]}

机构：

[1] Christian Med Coll Hosp, Dept Clin Virol, Vellore 632004, Tamil Nadu, India

[2] Christian Med Coll Hosp, Dept Internal Med, Unit 1, Vellore 632004, Tamil Nadu, India

[3] Christian Med Coll Hosp, Dept Dermatol, Vellore 632004, Tamil Nadu, India

[4] Christian Med Coll Hosp, Dept Clin Microbiol, Vellore 632004, Tamil Nadu, India

[5] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21205 USA

来源：

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY | 2000年 / 7卷 / 06期

关键词：

D O I：

10.1128/CDLI.7.6.987-989.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Nested PCRs For human immunodeficiency virus type 1 (HIV-1) and HIV-2 were compared with immunoblot test results. Twelve of 13 immunoblot-positive HIV-2 samples were positive by PCR, There were five INNO-LLA (Innogenetics, Zwijnaarde, Belgium) and/or HIVBLOT 2.2 (Genelabs, Singapore) samples that tested positive for dual infection. HIV-1 PCR was positive in all samples, while HIV-2 PCR was positive in two and RIBA (Chiron Corporation, San Diego, Calif.) was positive for HIV-2 in three samples. Thus the prevalence of HIV-2 is accurately estimated by the use of immunoblotting, hut that of HIV-1 and -2 dual infection may be overestimated.

引用

页码：987 / 989

页数：3

共 22 条

[11] Peripheral CD4+/CD8+T-lymphocyte counts estimated by an immunocapture method in the normal healthy south Indian adults and HIV seropositive individuals [J].

Kannangai, R ;

Prakash, KJ ;

Ramalingam, S ;

Abraham, OC ;

Mathews, KP ;

Jesudason, MV ;

Sridharan, G .

JOURNAL OF CLINICAL VIROLOGY, 2000, 17 (02) :101-108

[12] HIV-2 status in southern India [J].

Kannangai, R ;

Ramalingam, S ;

Castillo, RC ;

Babu, PG ;

John, TJ ;

Sridharan, G ;

Schwartz, DH .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1999, 93 (01) :30-31

[13]

KULKARNI S, 1992, INDIAN J MED RES-A, V95, P213

[14] CHARACTERIZATION OF DUAL HIV-1 AND HIV-2 SEROLOGICAL PROFILES BY POLYMERASE CHAIN-REACTION [J].

LEONARD, G ;

CHAPUT, A ;

COURGNAUD, V ;

SANGARE, A ;

DENIS, F ;

BRECHOT, C .

AIDS, 1993, 7 (09) :1185-1189

[15] VIROLOGICAL AND POLYMERASE CHAIN-REACTION STUDIES OF HIV-1 HIV-2 DUAL INFECTION IN COTE-DIVOIRE [J].

PEETERS, M ;

GERSHYDAMET, GM ;

FRANSEN, K ;

KOFFI, K ;

COULIBALY, M ;

DELAPORTE, E ;

PIOT, P ;

VANDERGROEN, G .

LANCET, 1992, 340 (8815) :339-340

[16]

PFUTZNER A, 1992, J ACQ IMMUN DEF SYND, V5, P972

[17] IDENTIFICATION OF MIXED HIV-1/HIV-2 INFECTIONS IN BRAZIL BY POLYMERASE CHAIN-REACTION [J].

PIENIAZEK, D ;

PERALTA, JM ;

FERREIRA, JA ;

KREBS, JW ;

OWEN, SM ;

SION, FS ;

CELSO, FR ;

SERENO, AB ;

DESA, CAM ;

WENIGER, BG ;

HEYWARD, WL ;

OU, CY ;

PIENIAZEK, NJ ;

SCHOCHETMAN, G ;

RAYFIELD, MA .

AIDS, 1991, 5 (11) :1293-1299

[18]

RICHARD D, 1994, AIDS, V8, P977

[19] SPREAD OF HIV-2 IN INDIA [J].

RUBSAMENWAIGMANN, H ;

BRIESEN, HV ;

MANIAR, JK ;

RAO, PK ;

SCHOLZ, C ;

PFUTZNER, A .

LANCET, 1991, 337 (8740) :550-551

[20]

RUBSAMENWAIGMANN H, 1994, ZBL BAKT-INT J MED M, V280, P398

← 1 2 3 →