Domestication of wild tomato is accelerated by genome editing

被引：456

作者：

Li, Tingdong ^{[1
,2
]}

Yang, Xinping ^{[3
,4
]}

Yu, Yuan ^{[2
,3
,4
]}

Si, Xiaomin ^{[1
,2
]}

Zhai, Xiawan ^{[3
,4
]}

Zhang, Huawei ^{[1
]}

Dong, Wenxia ^{[3
,4
]}

Gao, Caixia ^{[1
,2
]}

Xu, Cao ^{[2
,3
,4
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Genome Editing, State Key Lab Plant Cell & Chromosome Engn, Beijing, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, State Key Lab Plant Genom, Beijing, Peoples R China

[4] Chinese Acad Sci, Inst Genet & Dev Biol, CEPAMS, CAS JIC, Beijing, Peoples R China

来源：

NATURE BIOTECHNOLOGY | 2018年 / 36卷 / 12期

基金：

美国国家科学基金会;

关键词：

BACTERIAL SPOT; SHOOT; PIMPINELLIFOLIUM; RESISTANCE; GENETICS; WUSCHEL; TRAITS; SIZE; TOOL;

D O I：

10.1038/nbt.4273

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Crop improvement by inbreeding often results in fitness penalties and loss of genetic diversity. We introduced desirable traits into four stress-tolerant wild-tomato accessions by using multiplex CRISPR-Cas9 editing of coding sequences, cis-regulatory regions or upstream open reading frames of genes associated with morphology, flower and fruit production, and ascorbic acid synthesis. Cas9-free progeny of edited plants had domesticated phenotypes yet retained parental disease resistance and salt tolerance.

引用

页码：1160 / +

页数：6

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