Regulation of tissue β-adrenergic, glucocorticoid and androgen receptors induced by repeated exposure to growth promoters in male veal calves

被引：23

作者：

Odore, Rosangela ^{[1
]}

Badino, Paola ^{[1
]}

Barbero, Raffaella ^{[1
]}

Cuniberti, Barbara ^{[1
]}

Pagliasso, Susanna ^{[1
]}

Girardi, Carlo ^{[1
]}

Re, Giovanni ^{[1
]}

机构：

[1] Univ Turin, Dept Anim Pathol, Div Pharmacol & Toxicol, I-10095 Grugliasco, TO, Italy

来源：

RESEARCH IN VETERINARY SCIENCE | 2007年 / 83卷 / 02期

关键词：

calves; clenbuterol; dexamethasone; estradiol; growth promoters; receptors;

D O I：

10.1016/j.rvsc.2006.12.011

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Biochemical modifications induced by a combination of anabolic compounds in target organs of male veal calves have been evaluated. Six male Friesian crossbred calves were treated with of 17 beta-estradiol, dexamethasone sodium phosphate and clenbuterol or served as controls. P-Adrenoceptors (beta-ARs) were measured in myocardium, lung, spleen, cerebral cortex, hippocampus, thalamus, hypothalamus, and hypophysis, glucocorticoid receptors (GRs) in the spleen and androgen receptors (AnRs) in the testis, by binding assay. A significant decrease in beta-ARs was observed in all tissue samples from treated animals. In the spleen the two GR subtypes found, low (LA) and high (HA) affinity GRs, were down-regulated by the treatment. A significant (P < 0.05) decrease of testis weight and a significant (P < 0.05) up-regulation of AnRs was also observed. Our data demonstrate that long-term treatment with anabolic compounds markedly affects receptor concentrations in target organs of male veal calves. Thus, studies investigating biological assays as screening methods to detect such compounds should be encouraged. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：227 / 233

页数：7

共 32 条

[1] Possible role of dexamethasone in sensitizing the beta-2-adrenergic receptor system in vivo in calves during concomitant treatment with clenbuterol [J].

Abraham, G ;

Gottschalk, J ;

Ungemach, FR .

PHARMACOLOGY, 2004, 72 (03) :196-204

[2]

Adcock Ian M., 2002, Journal of Allergy and Clinical Immunology, V110, pS261, DOI 10.1067/mai.2002.129705

[3] Are so many adrenergic receptor subtypes really present in domestic animal tissues? A pharmacological perspective [J].

Badino, P ;

Odore, R ;

Re, G .

VETERINARY JOURNAL, 2005, 170 (02) :163-174

[4] Food poisoning by clenbuterol in Portugal [J].

Barbosa, J ;

Cruz, C ;

Martins, J ;

Silva, JM ;

Neves, C ;

Alves, C ;

Ramos, F ;

Da Silveira, MIN .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2005, 22 (06) :563-566

[5] GENITAL LESIONS FOLLOWING LONG-TERM ADMINISTRATION OF CLENBUTEROL IN FEMALE PIGS [J].

BIOLATTI, B ;

CASTAGNARO, M ;

BOLLO, E ;

APPINO, S ;

RE, G .

VETERINARY PATHOLOGY, 1994, 31 (01) :82-92

[6] Quantitative modification of the testicular structure in pigs fed with anabolic doses of clenbuterol [J].

Blanco, A ;

Artacho-Pérula, E ;

Flores-Acuña, R ;

Agüera, E ;

Monterde, JG .

VETERINARY RESEARCH, 2002, 33 (01) :47-53

[7]

Dacasto M., 1994, Medicina Veterinaria, V11, P561

[8]

DURSCH I, 1995, J ANIM SCI, V73, P2050

[9] Ligand-independent activation of the glucocorticoid receptor by β2-adrenergic receptor agonists in primary human lung fibroblasts and vascular smooth muscle cells [J].

Eickelberg, O ;

Roth, M ;

Lörx, R ;

Bruce, V ;

Rüdiger, J ;

Johnson, M ;

Block, LH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (02) :1005-1010

[10] IDENTIFICATION OF BETA-ADRENOCEPTORS IN RAT LYMPH-NODES AND SPLEEN - AN AUTORADIOGRAPHIC STUDY [J].

FERNANDEZLOPEZ, A ;

REVILLA, V ;

CANDELAS, MA ;

ALLER, MI ;

SORIA, C ;

PAZOS, A .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1994, 262 (03) :283-286

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