Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma

被引：19

作者：

Luk, JM

Su, YCF

Lam, SCT

Lee, CK

Hu, MY

He, QY

Lau, GK

Wong, FWS

Fan, ST

机构：

[1] Univ Hong Kong, Dept Surg, Hong Kong, Hong Kong, Peoples R China

[2] Univ Hong Kong, Ctr Study Liver Dis, Hong Kong, Hong Kong, Peoples R China

[3] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China

[4] Univ Hong Kong, Ctr Study Liver Dis, Dept Med, Hong Kong, Hong Kong, Peoples R China

[5] Fudan Univ, Zhongshan Hosp, Expt Res Ctr, Shanghai 200433, Peoples R China

[6] Natl Univ Singapore, Dept Pharmacol, Singapore 117548, Singapore

来源：

PROTEOMICS | 2005年 / 5卷 / 07期

关键词：

hepatocellular carcinoma; Ku autoantigen; monoclonal antibody; tumor marker;

D O I：

10.1002/pmic.200401084

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A murine monoclonal antibody (mAb), CLD3 (IgG(1),kappa), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix-assisted laser desorption/ionization-time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.

引用

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页码：1980 / 1986

页数：7

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