Heterogeneous nuclear ribonucleoprotein A2/B1 identified as an autoantigen in autoimmune hepatitis by proteome analysis

被引：37

作者：

Huguet, S

Labas, V

Duclos-Vallee, JC

Bruneel, A

Vinh, J

Samuel, D

Johanet, C

Ballot, E

机构：

[1] Hop St Antoine, Serv Immunol & Hematol Biol, F-75012 Paris, France

[2] Ecole Super Phys & Chim Ind Ville Paris, Lab Neurobiol & Divers Cellulaire, Paris, France

[3] Hop Paul Brousse, Ctr Hepatobiliaire, UPRES 1595, Villejuif, France

[4] Hop St Antoine, Serv Biochim A, AP HP, F-75012 Paris, France

来源：

PROTEOMICS | 2004年 / 4卷 / 05期

关键词：

antinuclear antibodies; autoantigen targeting; autoimmune hepatitis; mass spectrometry;

D O I：

10.1002/pmic.200300757

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Our aim was to characterize antinuclear antibody (ANA) targets in type 1 autoimmune hepatitis (AIH1) using a proteomic tool. ANA-positive sera from 29 patients with AIM and 14 negative controls were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The principal antigens identified were a 30 kDa triplet band which was recognized by 79% of patient sera and 14% of control sera, and a 36 kDa antigen which was recognized by 52% and 14% of sera, respectively. The latter antigen was studied in more detail using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Its identification corresponded to heterogeneous nuclear ribonucleoprotein A2/B1. This paper presents the first description of this protein as an antigen in AIH1.

引用

页码：1341 / 1345

页数：5

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