Determination of saikosaponins a, c, and d in Bupleurum Chinese DC from different areas by capillary zone electrophoresis

被引：37

作者：

Lin, XL

Xue, L

Zhang, HY

Zhu, CF

机构：

[1] Shandong Univ, Sch Chem & Chem Engn, Jinan 250100, Peoples R China

[2] Shandong Univ, Sch Pharm, Jinan 250012, Peoples R China

[3] Shandong Univ Tradit Chinese Med, Sch Chinese Pharm, Jinan 250014, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2005年 / 382卷 / 07期

关键词：

saikosaponins; Bupleurum Chinese DC; capillary zone electrophoresis;

D O I：

10.1007/s00216-005-3371-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A fast capillary zone electrophoresis (CZE) method was developed for the determination and separation of saikosaponins a, c, and d in Chinese herbal extracts of Bupleurum Chinese DC from different areas. Detection at 214 nm with a system containing sodium borate buffer and mono-3-phenylcarbamoyl-beta-CD was found to be the most suitable approach for this analysis. Saikosaponins a, c, and d could be easily determined within 8 min. The effect of the concentration of mono-3-phenylcarbamoyl-beta-CD, the concentration of the running buffer and buffer pH value on the migration behavior of the saikosaponins is discussed.

引用

页码：1610 / 1615

页数：6

共 27 条

[1]

Determination of saikosaponin derivatives in Radix bupleuri and in pharmaceuticals of the Chinese multiherb remedy Xiaochaihu-tang using liquid chromatographic tandem mass spectrometry [J].