Association of circulating miR-20a, miR-27a, and miR-126 with non-alcoholic fatty liver disease in general population

被引：42

作者：

Ando, Yoshitaka ^{[1
]}

Yamazaki, Mirai ^{[2
]}

Yamada, Hiroya ^{[3
]}

Munetsuna, Eiji ^{[4
]}

Fujii, Ryosuke ^{[5
]}

Mizuno, Genki ^{[6
]}

Ichino, Naohiro ^{[7
]}

Osakabe, Keisuke ^{[7
]}

Sugimoto, Keiko ^{[7
]}

Ishikawa, Hiroaki ^{[1
]}

Ohashi, Koji ^{[1
]}

Teradaira, Ryoji ^{[1
]}

Ohta, Yoshiji ^{[8
]}

Hamajima, Nobuyuki ^{[9
]}

Hashimoto, Shuji ^{[3
]}

Suzuki, Koji ^{[5
]}

机构：

[1] Fujita Hlth Univ, Sch Med Sci, Dept Biomed & Analyt Sci, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[2] Kagawa Prefectural Univ Hlth Sci, Dept Med Technol, 281-1 Murechohara, Takamatsu, Kagawa 7610123, Japan

[3] Fujita Hlth Univ, Sch Med, Dept Hyg, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[4] Fujita Hlth Univ, Sch Med, Dept Biochem, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[5] Fujita Hlth Univ, Sch Med Sci, Dept Prevent Med Sci, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[6] Fujita Hlth Univ Hosp, Dept Joint Res Lab Clin Med, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[7] Fujita Hlth Univ, Sch Med Sci, Dept Clin Physiol & Funct Imaging, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[8] Fujita Hlth Univ, Sch Med, Dept Chem, 1-98 Dengakugakubo,Kutsukake Cho, Toyoake, Aichi 4701192, Japan

[9] Nagoya Univ, Grad Sch Med, Dept Healthcare Adm, Showa Ku, 65 Tsurumai Cho, Nagoya, Aichi 4668550, Japan

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

MICRORNAS MIR-21; BIOMARKERS; NAFLD; EXPRESSION; FIBROSIS; PREDICT; DNA;

D O I：

10.1038/s41598-019-55076-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Non-alcoholic fatty liver disease (NAFLD) is closely associated with obesity, metabolic syndrome, and type II diabetes mellitus. Recently, circulating microRNAs (miRNAs) have been proposed as useful disease biomarkers. We examined whether circulating miRNAs, such as miR-20a, miR-27a, and miR-126, were useful biomarkers for NAFLD. We conducted a cross-sectional analysis of 527 subjects aged 39 years or older who had undergone a health examination in the Yakumo Study. Of the residents, 92 were diagnosed with NAFLD using a registered medical sonographer. Serum miR-20a, miR-27a and miR-126 levels were measured by quantitative real-time PCR. We then calculated the odds ratios for serum miRNA level changes according to the severity of NAFLD using normal liver status as the reference group. Serum levels of miR-20a and 27a, but not miR-126, were significantly lower in NAFLD subjects than normal subjects. Serum miR-20a and miR-27a levels were significantly lower in both male and female severe NAFLD subjects. Logistic regression analysis showed a significant relationship between low circulating miR-20a and 27a levels and severe NAFLD. Down-regulated circulating miR-20a and 27a levels were significantly associated with severe NAFLD in the general population. Circulating miR-20a and miR-27a may be useful biomarkers for severe NAFLD.

引用

页数：8

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