Determination of meropenem in serum by high-performance liquid chromatography with column switching

被引：30

作者：

Bompadre, S

Ferrante, L

De Martinis, M

Leone, L ^{[1
]}

机构：

[1] Univ Ancona, Fac Med, Inst Biomed Sci, I-60131 Ancona, Italy

[2] Univ Aquila, Dept Internal Med, I-67100 Laquila, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 812卷 / 1-2期

关键词：

column switching; sample preparation; meropenem; antibiotics;

D O I：

10.1016/S0021-9673(98)00249-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction for determination of meropenem in serum is described. Sample was directly injected onto the extraction column for sample clean-up and extraction. Thereafter, using an on-line column-switching system the drug was quantitatively transferred and separated on a C-18 analytical column. Ultraviolet absorption at 298 nm was used for detection. The assay was linear from 1 to 100 mu g/ml. Recovery was 98.5%. Based on a 20-mu l sample volume (serum-water, 1:1, v/v), detection limit was 0.1 mu g/ml. An application of the method to study the pharmacokinetics of meropenem is given. (C) 1998 Elsevier Science BN. All rights reserved.

引用

收藏

页码：249 / 253

页数：5

相关论文

共 12 条

[1] DETERMINATION OF MEROPENEM IN PLASMA BY HIGHPERFORMANCE LIQUID-CHROMATOGRAPHY AND A MICROBIOLOGICAL METHOD [J].

ALMESHAL, MA ;

RAMADAN, MA ;

LOTFI, KM ;

SHIBL, AM .

JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS, 1995, 20 (03) :159-163

[2] THE PHARMACOKINETICS OF MEROPENEM IN VOLUNTEERS [J].

BAX, RP ;

BASTAIN, W ;

FEATHERSTONE, A ;

WILKINSON, DM ;

HUTCHISON, M ;

HAWORTH, SJ .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1989, 24 :311-320

[3] Column-switching high-performance liquid chromatographic assay for minocycline of nude mice serum [J].

Bompadre, S ;

Ferrante, L ;

Leone, L ;

Montesi, M ;

Possati, L .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1997, 20 (08) :1257-1267

[4] ONLINE SOLID-PHASE EXTRACTION OF CEFTAZIDIME IN SERUM AND DETERMINATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BOMPADRE, S ;

FERRANTE, L ;

ALO, FP ;

LEONE, L .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1995, 669 (02) :265-269

[5]

BURMAN LA, 1991, J ANTIMICROB CHEMOTH, V27, P219

[6] COLUMN-SWITCHING TECHNIQUES FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF DRUGS IN BIOLOGICAL SAMPLES [J].

CAMPINSFALCO, P ;

HERRAEZHERNANDEZ, R ;

SEVILLANOCABEZA, A .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 619 (02) :177-190

[7] High-performance liquid chromatographic assay for meropenem in serum [J].

Elkhaili, H ;

Niedergang, S ;

Pompei, D ;

Linger, L ;

Leveque, D ;

Jehl, F .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1996, 686 (01) :19-26

[8] A COMPARISON OF THE PHARMACOKINETICS OF MEROPENEM AFTER ADMINISTRATION BY INTRAVENOUS-INJECTION OVER 5 MINUTES AND INTRAVENOUS-INFUSION OVER 30 MINUTES [J].

KELLY, HC ;

HUTCHISON, M ;

HAWORTH, SJ .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1995, 36 :35-41

[9] PHARMACOKINETICS OF MEROPENEM (ICI-194,660) AND ITS METABOLITE (ICI-213,689) IN HEALTHY-SUBJECTS AND IN PATIENTS WITH RENAL IMPAIRMENT [J].

LEROY, A ;

FILLASTRE, JP ;

BORSALEBAS, F ;

ETIENNE, I ;

HUMBERT, G .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1992, 36 (12) :2794-2798

[10] PHARMACOKINETICS OF MEROPENEM COMPARED TO IMIPENEM-CILASTATIN IN YOUNG, HEALTHY-MALES [J].

NILSSONEHLE, I ;

HUTCHISON, M ;

HAWORTH, SJ ;

NORRBY, SR .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1991, 10 (02) :85-88