yAP-1- and yAP-2-mediated, heat shock-induced transcriptional activation of the multidrug resistance ABC transporter genes in Saccharomyces cerevisiae

被引：41

作者：

Miyahara, K ^{[1
]}

Hirata, D ^{[1
]}

Miyakawa, T ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL,HIGASHIHIROSHIMA 739,JAPAN

来源：

CURRENT GENETICS | 1996年 / 29卷 / 02期

关键词：

heat-shock response; multidrug resistance; AP-1; homolog; Saccharomyces cerevisiae;

D O I：

10.1007/s002940050023

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have examined whether the stress-induced transcriptional activation of YDR1/PDR5/STS1 is mediated by yAP-1 and yAP-2. Of the stresses examined, heat shock-induced, rapid and transient PDR5 expression became very low in a yap1 yap2 double-gene disruptant, indicating that the yAP proteins mediate the response. Similar results were obtained with SNQ2, a close homologue of PDR5. A set of 5'-truncation derivatives of the PDR5 gene identified the region from -484 to -434 as being sufficient for the response. A sequence similar to the yAP-1 recognition element recently identified in the stress-responsive yeast genes was found in this region and in the 5'-flanking sequences of SNQ2.

引用

页码：103 / 105

页数：3