NDM-2 carbapenemase in Acinetobacter baumannii from Egypt

被引：181

作者：

Kaase, Martin ^{[2
]}

Nordmann, Patrice ^{[1
,3
]}

Wichelhaus, Thomas A. ^{[4
]}

Gatermann, Soeren G. ^{[2
]}

Bonnin, Remy A. ^{[1
,3
]}

Poirel, Laurent ^{[1
,3
]}

机构：

[1] Hop Bicetre, AP HP, Serv Bacteriol Virol,Fac Med, INSERM Emerging Resistance Antibiot U914, Paris, France

[2] Ruhr Univ Bochum, Dept Med Microbiol, Natl Reference Lab Multidrug Resistant Gram Negat, D-44801 Bochum, Germany

[3] Univ Paris 11, Paris, France

[4] Hosp Goethe Univ, Inst Med Microbiol & Infect Control, D-60596 Frankfurt, Germany

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2011年 / 66卷 / 06期

关键词：

metallo-beta-lactamases; Gram-negative; carbapenems; RESISTANCE; EMERGENCE; INDIA;

D O I：

10.1093/jac/dkr135

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: To analyse the mechanisms responsible for carbapenem resistance in one Acinetobacter baumannii isolate recovered from a patient transferred to Germany from an Egyptian hospital. Methods: PCR and sequencing were used to search for beta-lactamase and 16S RNA methylase genes. Multilocus sequence typing was used to determine the sequence type (ST) of the isolate. Results: Sequencing of the PCR product obtained using primers for bla(NDM-1) revealed a variant of NDM-1 that had a C to G substitution at position 82 resulting in an amino acid substitution of proline to alanine at position 28. This variant was designated NDM-2. Genes encoding extended-spectrum beta-lactamases or 16S RNA methylase were not detected. The strain lacked detectable plasmids and bla(NDM-2) was not transferred by conjugation. MLST showed that the isolate belonged to a new ST, ST103. Conclusions: This work further underlines the spread of NDM carbapenemases in A. baumannii, and the spread of the corresponding gene in the Middle East. It also describes the first variant of NDM-1.

引用

页码：1260 / 1262

页数：3

共 12 条

[11]

Poirel L, 2010, LANCET INFECT DIS, V10, P832, DOI 10.1016/S1473-3099(10)70279-6

[12] Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. [J].