A NASBA method to detect high- and low-pathogenicity H5 avian influenza viruses

被引：15

作者：

Collins, RA

Ko, LS

So, KL

Ellis, T

Lau, LT

Yu, ACH

机构：

[1] Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China

[2] Castle Peak Vet Lab, Agr Fisheries & Conservat Dept, Hong Kong, Peoples R China

[3] Hong Kong DNA Chips Ltd, Kowloon, Hong Kong, Peoples R China

来源：

AVIAN DISEASES | 2003年 / 47卷

关键词：

NASBA; avian influenza; H5; hemagglutinin; pathogenic; electrochemiluminescence;

D O I：

10.1637/0005-2086-47.s3.1069

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Nucleic acid sequence-based amplification (NASBA) allows the rapid amplification of specific regions of nucleic acid obtained from a diverse range of sources. It is especially suitable for amplifying RNA sequences. A NASBA technique was developed that allows the detection of avian influenza A subtype H5 from allantoic fluid harvested from inoculated chick embryos. The amplified viral RNA is detected by electrochemiluminescence. The described NASBA technique is a specific, rapid, and sensitive method of detection of influenza A subtype H5 viruses. More importantly, it can be used to distinguish high- and low-pathogenicity strains of the H5 subtype.

引用

页码：1069 / 1074

页数：6

共 8 条

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