An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression

被引：588

作者：

Neufert, Clemens ^{[1
]}

Becker, Christoph ^{[1
]}

Neurath, Markus F. ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Immunol Lab, Med Clin 1, D-55131 Mainz, Germany

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 08期

关键词：

D O I：

10.1038/nprot.2007.279

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Colorectal cancer is a life-threatening disease that can develop spontaneously or as a complication of inflammatory bowel diseases. Mouse models are essential tools for the preclinical testing of novel therapeutic options in vivo. Here, we provide a highly reliable protocol for an experimental mouse model to study the development of colon cancers. It is based on the mutagenic agent azoxymethane (AOM), which exerts colonotropic carcinogenicity. Repeated intraperitoneal administration of AOM results in the development of spontaneous tumors within 30 weeks. As an alternative option, inflammation-dependent tumor growth can be investigated by combining the administration of AOM with the inflammatory agent dextran sodium sulfate in drinking water, which causes rapid growth of multiple colon tumors per mouse within 10 weeks. Different scoring systems including number of tumors and tumor size identify factors promoting or inhibiting tumor initiation and/or tumor progression, respectively.

引用

页码：1998 / 2004

页数：7

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