Mammalian Genes Are Transcribed with Widely Different Bursting Kinetics

被引：679

作者：

Suter, David M. ^{[1
,2
]}

Molina, Nacho ^{[3
,4
]}

Gatfield, David ^{[1
,2
,5
]}

Schneider, Kim ^{[1
,2
]}

Schibler, Ueli ^{[1
,2
]}

Naef, Felix ^{[3
,4
]}

机构：

[1] Univ Geneva, Dept Mol Biol, CH-1211 Geneva, Switzerland

[2] Natl Ctr Competence Res Frontiers Genet, CH-1211 Geneva, Switzerland

[3] Ecole Polytech Fed Lausanne, Sch Life Sci, Inst Bioengn, CH-1015 Lausanne, Switzerland

[4] Swiss Inst Bioinformat, CH-1015 Lausanne, Switzerland

[5] Univ Lausanne, Ctr Integrat Genom, CH-1015 Lausanne, Switzerland

来源：

SCIENCE | 2011年 / 332卷 / 6028期

基金：

瑞士国家科学基金会; 欧洲研究理事会;

关键词：

EXPRESSION; CELLS; TIME;

D O I：

10.1126/science.1198817

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In prokaryotes and eukaryotes, most genes appear to be transcribed during short periods called transcriptional bursts, interspersed by silent intervals. We describe how such bursts generate gene-specific temporal patterns of messenger RNA (mRNA) synthesis in mammalian cells. To monitor transcription at high temporal resolution, we established various gene trap cell lines and transgenic cell lines expressing a short-lived luciferase protein from an unstable mRNA, and recorded bioluminescence in real time in single cells. Mathematical modeling identified gene-specific on-and off-switching rates in transcriptional activity and mean numbers of mRNAs produced during the bursts. Transcriptional kinetics were markedly altered by cis-regulatory DNA elements. Our analysis demonstrated that bursting kinetics are highly gene-specific, reflecting refractory periods during which genes stay inactive for a certain time before switching on again.

引用

页码：472 / 474

页数：3

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