Lymphocytes treated by extracorporeal photopheresis can down-regulate cytokine production in untreated monocytes

被引:20
作者
Bladon, J [1 ]
Taylor, PC [1 ]
机构
[1] Rotherham Gen Hosp, Dept Haematol, Rotherham S60 2UD, S Yorkshire, England
关键词
cytokines; graft-versus-host disease; monocytes; T cells;
D O I
10.1111/j.1600-0781.2005.00192.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background: Pro-inflammatory cytokines are actively involved in graft-versus host-disease (GvHD) aetiology. Treatment of GvHD, using extracorporeal photopheresis (ECP), has demonstrated clinical efficacy. ECP rapidly reduces the number of T cells that produce tumour necrosis factor alpha (TNF alpha), interferon gamma (IFN gamma) and interleukin (IL)2. ECP-treated cells are re-infused immediately after completion of treatment. This study attempted to determine the influence that ECP-treated cells would have on untreated cells following re-infusion. Methods: Heparinized samples were taken from 10 chronic GvHD patients, pre-ECP and immediately prior to re-infusion (post-ECP). Lymphocytes and monocytes were isolated using magnetic separation. The post-ECP lymphocytes were mixed with pre-ECP monocytes, while the post-ECP monocytes and pre-ECP lymphocytes were combined. After suitable stimulation, the T cells were tested for intracellular TNF alpha, IFN gamma and IL2, while the monocytes were evaluated for TNF alpha, IL1 alpha, IL1 beta, IL6 and IL8. Results: Although cytokine secretion is decreased in T cells exposed to ECP, pre-ECP T cells were unaffected by post-ECP monocytes. Post-ECP monocytes demonstrated a reduction in cytokine secretion. Furthermore, untreated monocytes down-regulated cytokine production following exposure to ECP-treated lymphocytes. Conclusion: ECP has both a direct and indirect immunosuppressive action, both of which may be beneficial in the treatment of GvHD.
引用
收藏
页码:293 / 302
页数:10
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