Determination of protein phosphorylation sites by mass spectrometry: a novel electrospray-based method

被引：8

作者：

Alverdi, V

Di Pancrazio, F

Lippe, G

Pucillo, C

Casetta, B

Esposito, G

机构：

[1] Univ Udine, Ctr Excellence MATI, Dept Biomed Sci & Technol, I-33100 Udine, Italy

[2] Appl Biosyst Inc, I-20052 Monza, Italy

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2005年 / 19卷 / 22期

关键词：

D O I：

10.1002/rcm.2198

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Several methods are used to identify protein phosphorylation sites. We report a novel electrospray-based method for the determination of phosphorylation sites by mass spectrometry, using two different declustering potential values. This method allows one to obtain, with a single liquid chromatography/mass spectrometry MOMS) run, the pattern with either the phosphorylated or the unphosphorylated species of a protein tryptic digest, that can be further analyzed by tracing back the origin of each HPO(3)-deprived form using the capabilities of tandem mass spectrometers. Copyright (c) 2005 John Wiley & Sons, Ltd.

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收藏

页码：3343 / 3348

页数：6

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