Peptidomics of the pars intercerebralis-corpus cardiacum complex of the migratory locust, Locusta migratoria

被引:119
作者
Clynen, E
Baggerman, G
Veelaert, D
Cerstiaens, A
Van der Horst, D
Harthoorn, L
Derua, R
Waelkens, E
De Loof, A
Schoofs, L
机构
[1] Catholic Univ Louvain, Inst Zool, B-3000 Louvain, Belgium
[2] Univ Utrecht, Dept Biochem Physiol, NL-3508 TC Utrecht, Netherlands
[3] Catholic Univ Louvain, Biochem Lab, B-3000 Louvain, Belgium
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 07期
关键词
Q-TOF mass spectrometric analysis; MALD-TOF imaging; Locusta migratoria; pars intercerebralis; corpora cardiaca;
D O I
10.1046/j.1432-1327.2001.02067.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pars intercerebralis-corpora cardiaca system (PI-CC) of insects is the endocrinological equivalent of the hypothalamus-pituitary system of vertebrates. Peptide profiles of the pars intercerebralis and the corpora cardiaca were characterized using simple sampling protocols in combination with MALDI-TOF and electrospray ionization double quadrupole time of flight (ESI-Qq-TOF) mass spectrometric technologies. The results were compared with earlier results of conventional sequencing methods and immunocytochemical methods. In addition to many known peptides, several m/z signals corresponding to putative novel peptides were observed in the corpora cardiaca and/or pars intercerebralis. Furthermore, for a number of peptides evidence was provided about their localization and MALDI-TOF analysis of the released material from the corpora cardiaca yielded information on the hormonal status of particular brain peptides.
引用
收藏
页码:1929 / 1939
页数:11
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