Differential Localization of Acid-Sensing Ion Channels 1 and 2 in Human Cutaneus Pacinian Corpuscles

被引：36

作者：

Calavia, M. G. ^{[1
]}

Montano, J. A. ^{[2
]}

Garcia-Suarez, O. ^{[1
]}

Feito, J. ^{[1
]}

Guervos, M. A. ^{[3
]}

Germana, A. ^{[4
,5
]}

del Valle, M. ^{[1
]}

Perez-Pinera, P. ^{[1
]}

Cobo, J. ^{[6
,7
]}

Vega, J. A. ^{[1
,8
]}

机构：

[1] Univ Oviedo, Dept Morfol & Biol Celular, Fac Med, E-33006 Oviedo, Spain

[2] Univ Catolica San Antonio, Dept Ciencias Salud, Murcia, Spain

[3] Univ Oviedo, Serv Cient Tecn, Serv Proceso Imagenes, E-33006 Oviedo, Spain

[4] Univ Messina, Fac Med Vet, Dipartimento MOBIFIPA, Sez Morfol, Messina, Italy

[5] Univ Messina, Fac Med Vet, CISS, Messina, Italy

[6] Univ Oviedo, Dept Cirugia & Especialidades Med Quirurg, E-33006 Oviedo, Spain

[7] Inst Asturiano Odontol, Oviedo, Spain

[8] Univ Oviedo, IUOPA, E-33006 Oviedo, Spain

来源：

CELLULAR AND MOLECULAR NEUROBIOLOGY | 2010年 / 30卷 / 06期

关键词：

ASIC proteins; Pacinian corpuscles; Immunohistochemistry; Human; Mechanoreceptors; GAMMA-SUBUNIT; BETA-SUBUNIT; EXPRESSION; ASIC2; ENAC; CONTRIBUTES; MECHANISMS; PROTEINS; MEISSNER; ENDINGS;

D O I：

10.1007/s10571-010-9511-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Acid-sensing ion channels (ASICs) are the members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily which mediate different sensory modalities including mechanosensation. ASICs have been detected in mechanosensory neurons as well as in peripheral mechanoreceptors. We now investigated the distribution of ASIC1, ASIC2, and ASIC3 proteins in human cutaneous Pacinian corpuscles using immunohistochemistry and laser confocal-scanner microscopy. We detected different patterns of expression of these proteins within Pacinian corpuscles. ASIC1 was detected in the central axon co-expressed with RT-97 protein, ASIC2 was expressed by the lamellar cells of the inner core co-localized with S100 protein, and ASIC3 was absent. These results demonstrate for the first time the differential distribution of ASIC1 and ASIC2 in human rapidly adapting low-threshold mechanoreceptors, and suggest specific roles of both proteins in mechanotransduction.

引用

页码：841 / 848

页数：8

共 41 条

[1]

A sensory neuron-specific, proton-gated ion channel [J].

Chen, CC ;

England, S ;

Akopian, AN ;

Wood, JN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10240-10245

[2]

Functional implications of the localization and activity of acid-sensitive channels in rat peripheral nervous system [J].

de la Rosa, DA ;

Zhang, P ;

Shao, D ;

White, F ;

Canessa, CM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2326-2331

[3]

Acid-sensing ion channels ASIC2 and ASIC3 do not contribute to mechanically activated currents in mammalian sensory neurones [J].

Drew, LJ ;

Rohrer, DK ;

Price, MP ;

Blaver, KE ;

Cockayne, DA ;

Cesare, P ;

Wood, JN .

JOURNAL OF PHYSIOLOGY-LONDON, 2004, 556 (03) :691-710

[4]

Localization of β and γ subunits of ENaC in sensory nerve endings in the rat foot pad [J].

Drummond, HA ;

Abboud, FM ;

Welsh, MJ .

BRAIN RESEARCH, 2000, 884 (1-2) :1-12

[5]

Eid S R, 2009, Handb Exp Pharmacol, P261, DOI 10.1007/978-3-540-79090-7_8

[6]

Genetics of sensory mechanotransduction [J].

Ernstrom, GG ;

Chalfie, M .

ANNUAL REVIEW OF GENETICS, 2002, 36 :411-453

[7]

Transport and localization of the DEG/ENaC ion channel BNaC1α to peripheral mechanosensory terminals of dorsal root ganglia neurons [J].

García-Añoveros, J ;

Samad, TA ;

Zuvela-Jelaska, L ;

Woolf, CJ ;

Corey, DR .

JOURNAL OF NEUROSCIENCE, 2001, 21 (08) :2678-2686

[8]

Molecular basis of mechanosensory transduction [J].

Gillespie, PG ;

Walker, RG .

NATURE, 2001, 413 (6852) :194-202

[9]

Immunohistochemical detection of ENaCβ in the terminal Schwann cells associated with the periodontal Ruffini endings of the rat incisor [J].

Hitomi, Yasumasa ;

Suzuki, Akiko ;

Kawano, Yoshiro ;

Nozawa-Inoue, Kayoko ;

Inoue, Makoto ;

Maeda, Takeyasu .

BIOMEDICAL RESEARCH-TOKYO, 2009, 30 (02) :113-119

[10]

Holzer Peter, 2009, Handb Exp Pharmacol, P283, DOI 10.1007/978-3-540-79090-7_9

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