Bacterial cytolethal distending toxin promotes the development of dysplasia in a model of microbially induced hepatocarcinogenesis

被引:131
作者
Ge, Zhongming [1 ]
Rogers, Arlin B. [1 ]
Feng, Yan [1 ]
Lee, Amy [1 ]
Xu, Shilu [1 ]
Taylor, Nancy S. [1 ]
Fox, James G. [1 ]
机构
[1] MIT, Div Comparat Med, Cambridge, MA 02139 USA
关键词
D O I
10.1111/j.1462-5822.2007.00939.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Bacterial cytolethal distending toxins (CDTs) containing DNase I-like activity can induce limited host DNA damage that leads to activation of the DNA-damage repair responses in cultured cell lines. However, in vivo experimental evidence linking CDTs to carcinogenesis is lacking. In this study, infection of A/JCr mice with an isogenic mutant of Helicobacter hepaticus lacking CDT activity (CDT mutant) induced chronic hepatitis comparable to wild-type H. hepaticus (Hh) infection at both 4 and 10 months post inoculation (MPI); however, the CDT mutant-infected mice did not develop hepatic dysplasic nodules at 10 MPI, whereas those infected with Hh did. There was no significant difference in hepatic colonization levels between the CDT mutant and Hh at both time points (P > 0.05). At 4 MPI, mice infected with Hh had significantly enhanced hepatic transcription of proinflammatory TNF-alpha, IFN-gamma and Cox-2, growth mediators IL-6 and TGF-alpha, anti-apoptotic Bcl-2 and Bcl-X-L, and increased hepatocyte proliferation (P < 0.05) compared with the control or the CDT mutant-infected mice. In addition, Hh infected male mice had upregulated hepatic mRNA levels of RelA (p65), p50, GADD45 beta and c-IAP1, components of the NF-kappa B pathway compared with the CDT mutant-infected mice. At 10 MPI, Hh infection was associated with significant upregulation of IL-6 mRNA. Activation of the inflammatory NF-kappa B pathway and upregulation of proinflammatory cytokines plus IL-6 in the Hh but not in the CDT mutant-infected mice suggest that Hh CDT plays a key role in promoting the dysplastic changes in Hh-infected mouse livers.
引用
收藏
页码:2070 / 2080
页数:11
相关论文
共 40 条
[21]   High susceptibility to bacterial infection, but no liver dysfunction, in mice compromised for hepatocyte NF-κB activation [J].
Lavon, I ;
Goldberg, I ;
Amit, S ;
Landsman, L ;
Jung, S ;
Tsuberi, BZ ;
Barshack, I ;
Kopolovic, J ;
Galun, E ;
Bujard, H ;
Ben-Neriah, Y .
NATURE MEDICINE, 2000, 6 (05) :573-577
[22]   Characterization of Haemophilus ducreyi cdtA, cdtB, and cdtC mutants in in vitro and in vivo systems [J].
Lewis, DA ;
Stevens, MK ;
Latimer, JL ;
Ward, CK ;
Deng, KP ;
Blick, R ;
Lumbley, SR ;
Ison, CA ;
Hansen, EJ .
INFECTION AND IMMUNITY, 2001, 69 (09) :5626-5634
[23]   IKKβ couples hepatocyte death to cytokine-driven compensatory proliferation that promotes chemical hepatocarcinogenesis [J].
Maeda, S ;
Kamata, H ;
Luo, JL ;
Leffert, H ;
Karin, M .
CELL, 2005, 121 (07) :977-990
[24]  
MAUAD TH, 1994, AM J PATHOL, V145, P1237
[25]   Gadd45β mediates the NF-κB suppression of JNK signalling by targeting MKK7/JNKK2 [J].
Papa, S ;
Zazzeroni, F ;
Bubici, C ;
Jayawardena, S ;
Alvarez, K ;
Matsuda, S ;
Nguyen, DU ;
Pham, CG ;
Nelsbach, AH ;
Melis, T ;
De Smaele, E ;
Tang, WJ ;
D'Adamio, L ;
Franzoso, G .
NATURE CELL BIOLOGY, 2004, 6 (02) :146-+
[26]   NF-κB functions as a tumour promoter in inflammation-associated cancer [J].
Pikarsky, E ;
Porat, RM ;
Stein, I ;
Abramovitch, R ;
Amit, S ;
Kasem, S ;
Gutkovich-Pyest, E ;
Urieli-Shoval, S ;
Galun, E ;
Ben-Neriah, Y .
NATURE, 2004, 431 (7007) :461-466
[27]   Modulation of host immune responses by the cytolethal distending toxin of Helicobacter hepaticus [J].
Pratt, Jason S. ;
Sachen, Kacey L. ;
Wood, Heather D. ;
Eaton, Kathryn A. ;
Young, Vincent B. .
INFECTION AND IMMUNITY, 2006, 74 (08) :4496-4504
[28]   Characterisation of cytolethal distending toxin (CDT) mutants of Campylobacter jejuni [J].
Purdy, D ;
Buswell, CM ;
Hodgson, AE ;
McAlpine, K ;
Henderson, I ;
Leach, SA .
JOURNAL OF MEDICAL MICROBIOLOGY, 2000, 49 (05) :473-479
[29]   Innate immune inflammatory response against enteric bacteria Helicobacter hepaticus induces mammary adenocarcinoma in mice [J].
Rao, Varada P. ;
Poutahidis, Theofilos ;
Ge, Zhongming ;
Nambiar, Prashant R. ;
Boussahmain, Chakib ;
Wang, Yan Yan ;
Horwitz, Bruce H. ;
Fox, James G. ;
Erdman, Susan E. .
CANCER RESEARCH, 2006, 66 (15) :7395-7400
[30]   Progression of chronic hepatitis and preneoplasia in Helicobacter hepaticus-infected A/JCr mice [J].
Rogers, AB ;
Boutin, SR ;
Whary, MT ;
Sundina, N ;
Ge, ZM ;
Cormier, K ;
Fox, JG .
TOXICOLOGIC PATHOLOGY, 2004, 32 (06) :668-677