An injectable extracellular matrix derived hydrogel for meniscus repair and regeneration

被引：160

作者：

Wu, Jinglei ^{[1
,2
]}

Ding, Qing ^{[1
,2
]}

Dutta, Ahana ^{[1
,2
]}

Wang, Yezhou ^{[1
,2
]}

Huang, Yi-hui ^{[1
,2
]}

Weng, Hong ^{[1
]}

Tang, Liping ^{[1
,2
]}

Hong, Yi ^{[1
,2
]}

机构：

[1] Univ Texas Arlington, Dept Bioengn, Arlington, TX 76019 USA

[2] Univ Texas SW Med Ctr Dallas, Joint Grad Biomed Engn Program, Dallas, TX 75390 USA

来源：

ACTA BIOMATERIALIA | 2015年 / 16卷

关键词：

Porcine meniscus; Decellularization; Extracellular matrix; Injectable hydrogel; Meniscus regeneration; TISSUE-ENGINEERING APPLICATIONS; PORCINE MEDIAL MENISCUS; RAT BONE-MARROW; IN-VITRO; COLLAGEN FIBRILLOGENESIS; CELL BEHAVIOR; SHEEP MODEL; STEM-CELLS; SCAFFOLD; CARTILAGE;

D O I：

10.1016/j.actbio.2015.01.027

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Tissue-derived extracellular matrix (ECM) biomaterials to regenerate the meniscus have gained increasing attention in treating meniscus injuries and diseases, particularly for aged persons and athletes. However, ECM scaffold has poor cell infiltration and can only be implanted using surgical procedures. To overcome these limitations, we developed an injectable ECM hydrogel material from porcine meniscus via modified decellularization and enzymatic digestion. This meniscus-derived ECM hydrogel exhibited a fibrous morphology with tunable compression and initial modulus. It had a good injectability evidenced by syringe injection into mouse subcutaneous tissue. The hydrogel showed good cellular compatibility by promoting the growth of both bovine chondrocytes and mouse 3T3 fibroblasts encapsulated in the hydrogel for 2 weeks. It also promoted cell infiltration as shown in both in vitro cell culture and in vivo mouse subcutaneous implantation. The in vivo study revealed that the ECM hydrogel possessed good tissue compatibility after 7 days of implantation. The results support the great potential of the newly produced injectable meniscus-derived ECM hydrogel specifically for meniscus repair and regeneration. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：49 / 59

页数：11

共 74 条

[51]

Substrate Modulus Directs Neural Stem Cell Behavior [J].

Saha, Krishanu ;

Keung, Albert J. ;

Irwin, Elizabeth F. ;

Li, Yang ;

Little, Lauren ;

Schaffer, David V. ;

Healy, Kevin E. .

BIOPHYSICAL JOURNAL, 2008, 95 (09) :4426-4438

[52]

Generation and characterization of a human acellular meniscus scaffold for tissue engineering [J].

Sandmann, G. H. ;

Eichhorn, S. ;

Vogt, S. ;

Adamczyk, C. ;

Aryee, S. ;

Hoberg, M. ;

Milz, S. ;

Imhoff, A. B. ;

Tischer, T. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2009, 91A (02) :567-574

[53]

Hydrogels derived from demineralized and decellularized bone extracellular matrix [J].

Sawkins, M. J. ;

Bowen, W. ;

Dhadda, P. ;

Markides, H. ;

Sidney, L. E. ;

Taylor, A. J. ;

Rose, F. R. A. J. ;

Badylak, S. F. ;

Shakeshehh, K. M. ;

White, L. J. .

ACTA BIOMATERIALIA, 2013, 9 (08) :7865-7873

[54]

Schwarz S, 2012, TISSUE ENG PT A, V18, P2195, DOI [10.1089/ten.tea.2011.0705, 10.1089/ten.TEA.2011.0705]

[55]

Healing of meniscal tissue by cellular fibrin glue: an in vivo study [J].

Scotti, C. ;

Pozzi, A. ;

Mangiavini, L. ;

Vitari, F. ;

Boschetti, F. ;

Domeneghini, C. ;

Fraschini, G. ;

Peretti, G. M. .

KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY, 2009, 17 (06) :645-651

[56]

Safety and Efficacy of an Injectable Extracellular Matrix Hydrogel for Treating Myocardial Infarction [J].

Seif-Naraghi, Sonya B. ;

Singelyn, Jennifer M. ;

Salvatore, Michael A. ;

Osborn, Kent G. ;

Wang, Jean J. ;

Sampat, Unatti ;

Kwan, Oi Ling ;

Strachan, G. Monet ;

Wong, Jonathan ;

Schup-Magoffin, Pamela J. ;

Braden, Rebecca L. ;

Bartels, Kendra ;

DeQuach, Jessica A. ;

Preul, Mark ;

Kinsey, Adam M. ;

DeMaria, Anthony N. ;

Dib, Nabil ;

Christman, Karen L. .

SCIENCE TRANSLATIONAL MEDICINE, 2013, 5 (173)

[57]

SILVER FH, 1981, J BIOL CHEM, V256, P4973

[58]

Catheter-Deliverable Hydrogel Derived From Decellularized Ventricular Extracellular Matrix Increases Endogenous Cardiomyocytes and Preserves Cardiac Function Post-Myocardial Infarction [J].

Singelyn, Jennifer M. ;

Sundaramurthy, Priya ;

Johnson, Todd D. ;

Schup-Magoffin, Pamela J. ;

Hu, Diane P. ;

Faulk, Denver M. ;

Wang, Jean ;

Mayle, Kristine M. ;

Bartels, Kendra ;

Salvatore, Michael ;

Kinsey, Adam M. ;

DeMaria, Anthony N. ;

Dib, Nabil ;

Christman, Karen L. .

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2012, 59 (08) :751-763

[59]

Injectable Materials for the Treatment of Myocardial Infarction and Heart Failure: The Promise of Decellularized Matrices [J].

Singelyn, Jennifer M. ;

Christman, Karen L. .

JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH, 2010, 3 (05) :478-486

[60]

Naturally derived myocardial matrix as an injectable scaffold for cardiac tissue engineering [J].

Singelyn, Jennifer M. ;

DeQuach, Jessica A. ;

Seif-Naraghi, Sonya B. ;

Littlefield, Robert B. ;

Schup-Magoffin, Pamela J. ;

Christman, Karen L. .

BIOMATERIALS, 2009, 30 (29) :5409-5416

← 1 2 3 4 5 6 7 8 →