Evidence for conserved tRNA genes in the 16S-23S rDNA spacer sequence and two rrn operons of Xylella fastidiosa

被引：6

作者：

Chen, JC ^{[1
]}

Banks, D

Jarret, RL

Jones, JB

机构：

[1] Florida A&M Univ, Ctr Viticulture Sci, Tallahassee, FL 32307 USA

[2] ARS, USDA, Dept Plant Genet Resources, Griffin, GA 30223 USA

[3] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2000年 / 46卷 / 12期

关键词：

Xylella fastidiosa; rrn operon numbers; 16S-23S rDNA spacer; tRNA;

D O I：

10.1139/cjm-46-12-1171

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 16S-23S rDNA spacer of the type strain (ATCC 35879) of Xylella fastidiosa was amplified by PCR, cloned, and sequenced. The spacer sequence (455 bp) contains two tRNA (tRNA(ala) and tRNA(ile)) genes. Identical tRNA genes were also found in the 16S-23S spacer sequences of all the 51 strains of X. fastidiosa retrieved from the GenBank database. At this particular locus, the gene order of tRNA(ala) - tRNA(ile) is conserved among all the studied strains of Xylella and Xanthomonas, and different from those of other bacteria. Sequence analysis showed that Xanthomonas is the most closely related genus. Results from restriction endonuclease analysis suggested the presence of two rrn operons in the genome of a Xylella fastidiosa Pierce's disease strain.

引用

页码：1171 / 1175

页数：5

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