High-performance liquid chromatography with ultraviolet detection for therapeutic drug monitoring of everolimus

被引：27

作者：

Baldelli, S

Murgia, S

Merlini, S

Zenoni, S

Perico, N

Remuzzi, G

Cattaneo, D

机构：

[1] Mario Negri Inst Pharmacol Res, Osped Riuniti Bergamo, Dept Med & Transplantat, I-24125 Bergamo, Italy

[2] Mario Negri Inst Pharmacol Res, Ctr Res Organ Transplantat Chiara Cucchi Alessand, I-24100 Bergamo, Italy

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 816卷 / 1-2期

关键词：

everolimus; validation; high-performance liquid chromatography;

D O I：

10.1016/j.jchromb.2004.11.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed and validated a high-performance liquid chromatography-ultraviolet (HPLC-UV) method for determining everolimus concentrations in human whole blood. Sample preparation involved a solid-phase extraction after protein precipitation. The separation of everolimus from internal standard (IS) and endogenous components was achieved using an isocratic elution on an octyl column. The method showed a linear relationship between peak height ratios and blood concentrations in the range of 1-200 ng/mL (r(2) = 0.9997). The observed intra- and inter-day assay imprecision had a coefficient of variation (CV) = 12.8%, and inaccuracy was 11.4%. The method was found to be precise, accurate, and sensible making it useful for routine therapeutic monitoring of everolimus. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：99 / 105

页数：7

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