Assessment of sirolimus concentrations in whole blood by high-performance liquid chromatography with ultraviolet detection

被引:26
作者
Cattaneo, D [1 ]
Perico, N [1 ]
Gaspari, F [1 ]
机构
[1] Osped Riuniti Bergamo, Mario Negri Inst Pharmacol Res, Dept Immunol & Clin Organ Transplantat, I-24125 Bergamo, Italy
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 774卷 / 02期
关键词
sirolimus;
D O I
10.1016/S1570-0232(02)00204-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel, reversed-phase high-performance liquid chromatographic (HPLC) method is described for the analysis of sirolimus (SRL) in whole blood. The samples were purified by precipitating blood matrix with zinc sulfate, SRL was then extracted with acetone followed by solid-phase extraction. The method was linear over a range of 1-100 ng/ml and the lower limit of quantification was 2.5 ng/ml. The coefficient of variation (within day) was below 8.0% for the lowest SRL concentration. The day-to-day coefficient of variation was below 6.6%. The assay did not show interference peaks with immunosuppressive drugs commonly given to transplant patients. With the simplified extraction procedure described, 60 samples (including controls and calibration curve) can be quantified in a day. The sensitivity and rapidity of this analytical procedure makes it useful for routine therapeutic monitoring of SRL. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:187 / 194
页数:8
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