HLA-C high resolution typing: analysis of exons 2 and 3 by sequence based typing and detection of polymorphisms in exons 1-5 by sequence specific primers

被引:21
作者
Delfino, L [1 ]
Morabito, A [1 ]
Longo, A [1 ]
Ferrara, GB [1 ]
机构
[1] IST, Natl Canc Inst, Adv Biotechnol Ctr, Genoa, Italy
来源
TISSUE ANTIGENS | 1998年 / 52卷 / 03期
关键词
HLA-C; PCR-SBT; PCR-SSP; serological typing;
D O I
10.1111/j.1399-0039.1998.tb03040.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
HLA-C high resolution sequence based typing developed in this study involves a unique DNA amplification encompassing exon 1 to intron 3 and four fluorescent sequencing reactions covering exon 2 and 3. Both dye primer and dye terminator sequencing techniques were performed and results compared. This approach allowed the identification of all of the 50 HLA-C allelic variants so far described, except for two allele pairs that are distinguished by non-coding nucleotide changes (Cw*12021=12022, Cw*15051 = 15052) and three allele pairs (Cw*0701 = 0706, Cw*1701 = 1702 and Cw*1801 = 1802) that share the same nucleotide sequence in exon 2 and 3. For complete subtyping of these allelic variants, an amplification based on sequence specific primers (PCR-SSP) was used. No ambiguous heterozygous combinations of alleles were detected in our panel so far. HLA-C typing data obtained by this method were compared with data from serological and low resolution PCR-SSP typing, which had been performed previously on the samples sequenced.
引用
收藏
页码:251 / 259
页数:9
相关论文
共 44 条
[1]  
AIZAWA M, 1986, ASIA OCEANIA, P1079
[2]   REJECTION OF A KIDNEY GRAFT MISMATCHED ONLY FOR THE HLA-C LOCUS AND AN HLA-BW22 SPLIT [J].
BAAN, CC ;
VAESSEN, LMB ;
TENKATE, F ;
SCHREUDER, GMT ;
CLAAS, FHJ ;
WEIMAR, W ;
JUTTE, NHPM .
TRANSPLANTATION, 1993, 55 (02) :438-439
[3]   A correlation between HLA-C matching and donor antirecipient CTL precursor frequency in bone marrow transplantation [J].
Barnardo, MCNM ;
Davey, NJ ;
Bunce, M ;
Brookes, PA ;
Lechler, RI ;
Welsh, KI ;
Batchelor, JR .
TRANSPLANTATION, 1996, 61 (09) :1420-1423
[4]  
BAUR MP, 1984, HISTOCOMPATIBILITY T, P677
[5]  
BETTINOTTI MP, 1997, FUNCTIONAL MED IMPLI, V2, P373
[6]  
BJORKMAN PJ, 1990, ANNU REV BIOCHEM, V59, P253, DOI 10.1146/annurev.biochem.59.1.253
[7]   Nomenclature for factors of the HLA system, 1996 [J].
Bodmer, JG ;
Marsh, SGE ;
Albert, ED ;
Bodmer, WF ;
Bontrop, RE ;
Charron, D ;
Dupont, B ;
Erlich, HA ;
Fauchet, R ;
Mach, B ;
Mayr, WR ;
Parham, P ;
Sasazuki, T ;
Schreuder, GMT ;
Strominger, JL ;
Svejgaard, A ;
Terasaki, PI .
TISSUE ANTIGENS, 1997, 49 (03) :297-321
[8]   RAPID DNA TYPING FOR HLA-C USING SEQUENCE-SPECIFIC PRIMERS (PCR-SSP) - IDENTIFICATION OF SEROLOGICAL AND NON-SEROLOGICALLY DEFINED HLA-C ALLELES INCLUDING SEVERAL NEW ALLELES [J].
BUNCE, M ;
WELSH, KI .
TISSUE ANTIGENS, 1994, 43 (01) :7-17
[9]  
Bunce M., 1997, Tissue Antigens, V50, P100, DOI 10.1111/j.1399-0039.1997.tb02847.x
[10]  
CHAPMAN JR, 1986, TRANSPLANTATION, V42, P91