Hydroxysafflor yellow A induces autophagy in human liver cancer cells by regulating Beclin 1 and ERK expression

被引：32

作者：

Chen, Ziwei ^{[1
]}

Liu, Li ^{[2
]}

Liu, Yueyun ^{[1
]}

Wang, Shuyan ^{[1
]}

Zhang, Shujing ^{[1
]}

Dong, Ruijuan ^{[1
]}

Xu, Mingyang ^{[1
]}

Ma, Yicong ^{[1
]}

Wang, Jingjing ^{[3
]}

Zhang, Qian ^{[1
]}

Wei, Peng ^{[1
]}

机构：

[1] Beijing Univ Chinese Med, Sch Tradit Chinese Med, 11 North Third Ring Rd East, Beijing 100029, Peoples R China

[2] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China

[3] Anyang Hosp Tradit Chinese Med, Oncol Microstart Intervent Dept, Anyang 455001, Henan, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2020年 / 19卷 / 04期

基金：

中国国家自然科学基金;

关键词：

hydroxysafflor yellow A; autophagy; light chain 3; Beclin; 1; electron microscopy; PROTECTIVE AUTOPHAGY; APOPTOSIS; LC3;

D O I：

10.3892/etm.2020.8552

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Hydroxysafflor yellow A (HSYA) is a water-soluble component of the safflower (Carthamus tinctorius), and research has revealed that HSYA exhibits antitumor effects. In the present study, the effects of HSYA on the autophagy of a Hep-G2 liver cancer cell line, as well as the underlying mechanisms, were investigated. Hep-G2 cells were treated with HSYA and the viability of cells was measured using an MTT assay. Western blotting and immunofluorescence assays were performed to determine the expression of light chain 3 II (LC3-II) and p62, as well as the autophagy regulators Beclin 1 and ERK1/2. Transmission electron microscopy was performed to observe the formation of autophagosomes. The combined effects of HSYA and the autophagy inhibitor chloroquine (CQ) were also determined. The results revealed that the viability of Hep-G2 cells decreased with increasing concentrations of HSYA. Furthermore, LC3-II expression increased significantly and the level of p62 decreased significantly in the HYSA group compared with the control group. Additionally, an increase in Beclin 1 expression and a decrease in phosphorylated-ERK1/2 expression was observed in Hep-G2 cells treated with HYSA. Following treatment with CQ and HSYA, a significant increase in the viability of Hep-G2 cells was observed compared with the HSYA group. Collectively, the results indicated that HSYA induced autophagy by promoting the expression of Beclin 1 and inhibiting the phosphorylation of ERK in liver cancer cells. Therefore, HSYA may serve as a potential therapeutic agent for liver cancer.

引用

页码：2989 / 2996

页数：8

共 38 条

[21]

Targeting the WEE1 kinase strengthens the antitumor activity of imatinib via promoting KIT autophagic degradation in gastrointestinal stromal tumors [J].