Potential use of the comet assay in the clinical management of cancer

被引：88

作者：

McKenna, Declan J. ^{[1
]}

McKeown, Stephanie R. ^{[1
]}

McKelvey-Martin, Valerie J. ^{[1
]}

机构：

[1] Univ Ulster, Canc & Ageing Res Grp, Biomed Sci Res Inst, Coleraine BT52 1SA, Londonderry, North Ireland

来源：

MUTAGENESIS | 2008年 / 23卷 / 03期

关键词：

D O I：

10.1093/mutage/gem054

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The comet assay has been widely used to measure a range of cellular responses to DNA damage and has found applications in genotoxicity studies, bio-monitoring, ecological testing and in the study of human disease. This review discusses how the comet assay has been applied to the study of DNA damage and repair associated with cancer. The potential of the assay as a tool for predicting an individual's tumour sensitivity to radiation and to various chemotherapeutic drugs is examined, as well as outlining the usefulness of the assay in assessing oxidative stress within tumours. In addition, we review the use of the comet assay in investigations of the DNA-damaging effect of anti-neoplastic drugs and radiation used during cancer therapy. The advantages and limitations of the comet assay in carrying out all these studies are outlined, and the suitability of the comet assay for use in the clinical management of cancer is discussed.

引用

页码：183 / 190

页数：8

共 104 条

[71] Risk factors, DNA damage, and disease progression in Barrett's esophagus [J].

Olliver, JR ;

Hardie, LJ ;

Gong, Y ;

Dexter, S ;

Chalmers, D ;

Harris, KM ;

Wild, CP .

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2005, 14 (03) :620-625

[72] In vitro radiosensitivity measured in lymphocytes and fibroblasts by colony formation and comet assay:: comparison with clinical acute reactions to radiotherapy in breast cancer patients [J].

Oppitz, U ;

Schulte, S ;

Stopper, H ;

Baier, K ;

Müller, M ;

Wulf, J ;

Schakowski, R ;

Flentje, M .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2002, 78 (07) :611-616

[73]

Palyvoda O, 2003, ACTA BIOCHIM POL, V50, P181

[74] PLATINUM DNA DAMAGE IN LEUKOCYTE DNA OF PATIENTS RECEIVING CARBOPLATIN AND CISPLATIN CHEMOTHERAPY, MEASURED BY ATOMIC-ABSORPTION SPECTROMETRY [J].

PARKER, RJ ;

GILL, I ;

TARONE, R ;

VIONNET, JA ;

GRUNBERG, S ;

MUGGIA, FM ;

REED, E .

CARCINOGENESIS, 1991, 12 (07) :1253-1258

[75] POTENTIAL METHODS FOR PREDICTING TUMOR RADIOCURABILITY [J].

PETERS, LJ ;

BROCK, WA ;

JOHNSON, T ;

MEYN, RE ;

TOFILON, PJ ;

MILAS, L .

INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1986, 12 (04) :459-467

[76]

Pfuhler S, 1996, ENVIRON MOL MUTAGEN, V27, P196, DOI 10.1002/(SICI)1098-2280(1996)27:3<196::AID-EM4>3.0.CO

[77]

2-D

[78] Temozolomide pharmacodynamics in patients with metastatic melanoma: DNA damage and activity of repair enzymes 6-alkylguanine alkyltransferase and poly(ADP-ribose) polymerase-1 [J].

Plummer, ER ;

Middleton, MR ;

Jones, C ;

Olsen, A ;

Hickson, I ;

McHugh, P ;

Margison, GP ;

McGown, G ;

Thorncroft, M ;

Watson, AJ ;

Boddy, AV ;

Calvert, AH ;

Harris, AL ;

Newell, DR ;

Curtin, NJ .

CLINICAL CANCER RESEARCH, 2005, 11 (09) :3402-3409

[79]

Price ME, 2000, RADIAT RES, V153, P788, DOI 10.1667/0033-7587(2000)153[0788:IARODD]2.0.CO

[80]

← 2 3 4 5 6 7 8 9 10 11 →