Proanthocyanidin-rich extract from grape seeds attenuates the development of aortic atherosclerosis in cholesterol-fed rabbits

被引:308
作者
Yamakoshi, J
Kataoka, S
Koga, T
Ariga, T
机构
[1] Kikkoman Foods Inc, Div Res & Dev, Noda, Chiba 278, Japan
[2] Noda Inst Sci Res, Noda, Chiba 278, Japan
关键词
proanthocyanidins; grape seed extract; antioxidant; atherosclerosis;
D O I
10.1016/S0021-9150(98)00230-5
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The aim of this study was to evaluate the antiatherosclerotic effect of proanthocyanidin-rich extracts from grape seeds in cholesterol-fed rabbits. Proanthocyanidin-rich extracts (0.1% and 1% in diets [w/w]) did not appreciably affect the changes in serum lipid profile of cholesterol-fed rabbits. The level of cholesteryl ester hydroperoxides (ChE-OOH) induced by 2,2'-azobis(2-amidinopropane-dihydrochloride (AAPH) were lower in the plasma of rabbits fed proanthocyanidin-rich extract plus cholesterol than in the plasma of rabbits fed cholesterol alone, but not in the low-density lipoprotein (LDL). Aortic malondialdehyde (MDA) content decreased in rabbits fed proanthocyanidin-rich extract. Feeding proanthocyanidin-rich extracts (0.1 and 1% in the diet) to rabbits significantly reduced severe atherosclerosis in the aorta. Immunohistochemical analysis revealed a decrease in the number of oxidized LDL-positive macrophage-derived foam cells in atherosclerotic lesions in the aorta of rabbits fed proanthocyanidin-rich extract. When proanthocyanidin-rich extract was administered orally to rats, proanthocyanidin was detected in the plasma by Porters method but not in the lipoproteins (LDL plus VLDL). In an in vitro experiment using human plasma, proanthocyanidin-rich extract added to the plasma inhibited the oxidation of cholesteryl linoleate in LDL, but not in the LDL isolated after the plasma and the extract were incubated in advance. These results suggested that proanthocyanidins, the major polyphenols in red wine, might trap reactive oxygen species in aqueous series such as plasma and interstitial fluid of the arterial wall, thereby inhibiting oxidation of LDL and showing an antiatherosclerotic activity. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:139 / 149
页数:11
相关论文
共 39 条
[11]   CONSUMPTION OF RED WINE WITH MEALS REDUCES THE SUSCEPTIBILITY OF HUMAN PLASMA AND LOW-DENSITY-LIPOPROTEIN TO LIPID-PEROXIDATION [J].
FUHRMAN, B ;
LAVY, A ;
AVIRAM, M .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1995, 61 (03) :549-554
[12]  
HARMAND MF, 1978, EUR J DRUG METAB PH, V1, P15
[13]   DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM [J].
HAVEL, RJ ;
EDER, HA ;
BRAGDON, JH .
JOURNAL OF CLINICAL INVESTIGATION, 1955, 34 (09) :1345-1353
[14]   Delapril slows the progression of atherosclerosis and maintains endothelial function in cholesterol-fed rabbits [J].
Hernandez, A ;
Barberi, L ;
Ballerio, R ;
Testini, A ;
Ferioli, R ;
Bolla, M ;
Natali, M ;
Folco, G ;
Catapano, AL .
ATHEROSCLEROSIS, 1998, 137 (01) :71-76
[15]  
HOLMAN RL, 1958, LAB INVEST, V7, P42
[16]  
ITABE H, 1994, J BIOL CHEM, V269, P15274
[17]  
KLEINVELD HA, 1992, CLIN CHEM, V38, P2066
[18]  
LAPARRA J, 1977, J PLANT MED PHYTOTHE, V11, P133
[19]   CONJUGATED LINOLEIC-ACID AND ATHEROSCLEROSIS IN RABBITS [J].
LEE, KN ;
KRITCHEVSKY, D ;
PARIZA, MW .
ATHEROSCLEROSIS, 1994, 108 (01) :19-25
[20]  
LOPESVIRELLA MF, 1977, CLIN CHEM, V23, P882