Effect of IGL-1, a new preservation solution, on kidney grafts (a pre-clinical study)

被引:27
作者
Badet, L
Ben Abdennebi, H
Petruzzo, P
McGregor, B
Espa, M
Hadj-Aissa, A
Ramella-Virieux, S
Steghens, JP
Portoghese, F
Martin, X
机构
[1] Univ Lyon 1, Hop Edouard Herriot, Serv Urol & Transplantat, F-69437 Lyon, France
[2] Fac Pharm, Lab Physiol Humaine, Monastir, Tunisia
[3] Univ Cagliari, Dept Surg & Transplantat, Cagliari, Italy
[4] Fdn Georges Lopez, Lyon, France
关键词
ischemia-reperfusion injury; preservation solution; extracellular solution; polyethylene glycol; kidney transplantation;
D O I
10.1111/j.1432-2277.2004.tb00516.x
中图分类号
R61 [外科手术学];
学科分类号
摘要
Ischemia-reperfusion injury conditions short-term and long-term graft function. The effects of the inversion of K+ and Na+ concentrations and substitution with polyethylene glycol for hydroxyethyl starch in University of Wisconsin (K-UW) solution were evaluated in isolated perfused rat kidneys and in autotransplanted pig kidneys. In the rat model kidneys were cold-stored for 24 h in K-UW or Na-UW or Na-PEG UW solutions (IGL-1 solution). Fractional sodium reabsorption and glomerular filtration rate was better in kidneys preserved in Na-UW and IGL-1 solution than those preserved in K-UW solution. In the pig model the left kidney was harvested and preserved in K-UW or IGL-1 solution for 24 It and then transplanted. In the autotransplanted pig model, kidneys preserved in IGL-1 solution showed a better function and a significant reduction of MHC class II expression, cellular apoptosis and interstitial fibrosis. In conclusion, kidneys preserved in IGL-1 solution tolerated ischemia/ reperfusion injury better than those preserved in K-UW solution.
引用
收藏
页码:815 / 821
页数:7
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