Determination of subyoctomole amounts of nonfluorescent molecules using a thermal lens microscope: Subsingle molecule determination

被引:182
作者
Tokeshi, M
Uchida, M
Hibara, A
Sawada, T
Kitamori, T
机构
[1] Kanagawa Acad Sci & Technol, Integrated Chem Project, Takatsu Ku, Kawasaki, Kanagawa 2130012, Japan
[2] Univ Tokyo, Grad Sch Engn, Dept Appl Chem, Bunkyo Ku, Tokyo 1138656, Japan
[3] Japan Sci & Technol Corp, Precursory Res Embryon Sci & Technol, Tokyo, Japan
关键词
D O I
10.1021/ac001479g
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The photothermal effect of an ultratrace amount of non fluorescent molecules in liquid was determined optimizing the optical arrangement for a thermal lens microscope, The optimized experimental setup could be determined from the evaluation of probing volume and the concentration of the sample solutions even when the expectation of the molecule number in the probing region was less than a single molecule. The minimum expectation, which is explained as being the time average, was 0.4 molecule of Pb(II) octaethylporphyrin (OEP) In benzene. The concentrations in the 9.7 x 10(-11)-7.8 x 10(-10) M region used in this work corresponded to the expected number of 0.4 -3.4 molecules, and the calibration curve in this region showed good linearity. Taking into account the enhancement factor of solvent, the molar absorption coefficient of solute, and the optimization of the optical arrangement, the present result, which was the determination limit of 0.34, was consistent with that previously reported. The relation between molecular behavior in the probing volume and the signal was discussed, The average temperature rise in the probing volume by the photothermal effect for the single OEP molecule was estimated as 3.1 muK and this value nas detectable, based on conventional thermal lens measurements for bulk scale sample.
引用
收藏
页码:2112 / 2116
页数:5
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