A leucine-rich repeat protein of carrot that exhibits antifreeze activity

被引：95

作者：

Meyer, K ^{[1
]}

Keil, M

Naldrett, MJ

机构：

[1] Shell Int Renewables, Forestry Res Unit, W Malling ME19 6BJ, England

[2] John Innes Ctr Plant Sci Res, Nitrogen Fixat Lab, Norwich NR4 7UH, Norfolk, England

来源：

FEBS LETTERS | 1999年 / 447卷 / 2-3期

关键词：

leucine-rich repeat protein; polygalacturonase inhibitor protein; antifreeze protein; apoplast; Arabidopsis thaliana; Daucus carota;

D O I：

10.1016/S0014-5793(99)00280-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene is rapidly induced by low temperatures. Furthermore, expression of the AFP gene in transgenic Arabidopsis thaliana plants leads to an accumulation of antifreeze activity. Our findings suggest that a new type of plant antifreeze protein has recently evolved from PGIPs, (C) 1999 Federation of European Biochemical Societies.

引用

页码：171 / 178

页数：8

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