The recently discovered 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway for the biosynthesis of plastid isoprenoids (including carotenoids) is not fully elucidated yet despite its central importance for plant life. It is known, however, that the first reaction completely specific to the pathway is the conversion of 1-deoxy-d-xylulose 5-phosphate (DXP) into MEP by the enzyme DXP reductoisomerase (DXR). We have identified a tomato cDNA encoding a protein with homology to DXR and in vivo activity, and show that the levels of the corresponding DXR mRNA and encoded protein in fruit tissues are similar before and during the massive accumulation of carotenoids characteristic of fruit ripening. The results are consistent with a non-limiting role of DXR, and support previous work proposing DXP synthase (DXS) as the first regulatory enzyme for plastid isoprenoid biosynthesis in tomato fruit. Inhibition of DXR activity by fosmidomycin showed that plastid isoprenoid biosynthesis is required for tomato fruit carotenogenesis but not for other ripening processes. In addition, dormancy was reduced in seeds from fosmidomycin-treated fruit but not in seeds from the tomato yellow ripe mutant (defective in phytoene synthase-1, PSY1), suggesting that the isoform PSY2 might channel the production of carotenoids for abscisic acid biosynthesis. Furthermore, the complete arrest of tomato seedling development using fosmidomycin confirms a key role of the MEP pathway in plant development.
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Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, CanadaNatl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
Cutler, AJ
Krochko, JE
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Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, CanadaNatl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Fraser, PD
Kiano, JW
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机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Kiano, JW
Truesdale, MR
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机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Truesdale, MR
Schuch, W
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机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Schuch, W
Bramley, PM
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Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, EnglandUniv London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
机构:
Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, CanadaNatl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
Cutler, AJ
Krochko, JE
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h-index: 0
机构:
Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, CanadaNatl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Fraser, PD
Kiano, JW
论文数: 0引用数: 0
h-index: 0
机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Kiano, JW
Truesdale, MR
论文数: 0引用数: 0
h-index: 0
机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Truesdale, MR
Schuch, W
论文数: 0引用数: 0
h-index: 0
机构:Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England
Schuch, W
Bramley, PM
论文数: 0引用数: 0
h-index: 0
机构:
Univ London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, EnglandUniv London Royal Holloway & Bedford New Coll, Sch Biol Sci, Div Biochem, Egham TW20 0EX, Surrey, England