Saccharification of corn fiber using enzymes from Aureobasidium sp strain NRRL Y-2311-1

被引：31

作者：

Leathers, TD

Gupta, SC

机构：

[1] Biopolymer Research Unit, National Center for Agricultural Utilization Research (NCAUR), US Dept. of Agriculture, Peoria

[2] Biologies, Biotechnology and Environmental Protection, Animal and Plant Health Inspection Service, US Dept. of Agriculture, Riverdale, MD

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1996年 / 59卷 / 03期

关键词：

corn fiber; enzymatic hydrolysis; hemicellulose; saccharification; xylanase;

D O I：

10.1007/BF02783575

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crude enzyme preparations from Aureobasidium sp. strain NRRL Y-2311-1 were characterized and tested for the capacity to saccharify corn fiber. Cultures grown on xylan, corn fiber, and alkaline hydrogen peroxide (AHP)-pretreated corn fiber produced specific levels of endoxylanase, amylase, protease, cellulase, and other activities. Using equal units of endoxylanase activity, crude enzymes from AHP-pretreated corn fiber cultures were most effective in saccharification. Multiple enzyme activities were implicated in this process. Pretreatment of corn fiber with AHP nearly doubled the susceptibility of hemicellulose to enzymatic digestion. Up to 138 mg xylose, 125 mg arabinose, and 490 mg glucose were obtained per g pretreated corn fiber under conditions tested.

引用

页码：337 / 347

页数：11