Tunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot

被引：68

作者：

Dash, Biraja C. ^{[1
]}

Mahor, Sunil ^{[1
]}

Carroll, Oliver ^{[1
]}

Mathew, Asha ^{[1
]}

Wang, Wenxin ^{[1
]}

Woodhouse, Kimberly A. ^{[2
]}

Pandit, Abhay ^{[1
]}

机构：

[1] Natl Univ Ireland, Network Excellence Funct Biomat NFB, Galway, Ireland

[2] Queens Univ, Dept Chem Engn, Kingston, ON K7L 3N6, Canada

来源：

JOURNAL OF CONTROLLED RELEASE | 2011年 / 152卷 / 03期

基金：

爱尔兰科学基金会;

关键词：

Self-assembly; Monodispersed; Tunable; Elastin-like polypeptide; Hollow sphere and transfection; EXPRESSED HUMAN ELASTIN; MICROBIAL TRANSGLUTAMINASE; TRANSFECTION EFFICIENCY; IN-VITRO; DNA; BIOCOMPATIBILITY; NANOSTRUCTURES; MICROPARTICLES; NANOPARTICLES; BIOMATERIAL;

D O I：

10.1016/j.jconrel.2011.03.006

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Self-assembly driven processes can be utilized to produce a variety of nanostructures useful for various in vitro and in vivo applications. Characteristics such as size, stability, biocompatibility, high therapeutic loading and controlled delivery of these nanostructures are particularly crucial in relation to in vivo applications. In this study, we report the fabrication of tunable monodispersed elastin-like polypeptide (ELP) hollow spheres of 100, 300, 500 and 1000 nm by exploiting the self-assembly property and net positive charge of ELP. The microbial transglutaminase (mTGase) cross-linking provided robustness and stability to the hollow spheres while maintaining surface functional groups for further modifications. The resulting hollow spheres showed a higher loading efficiency of plasmid DNA (pDNA) by using polyplex (similar to 70 mu g pDNA/mg of hollow sphere) than that of self-assembled ELP particles and demonstrated controlled release triggered by protease and elastase. Moreover, polyplex-loaded hollow spheres showed better cell viability than polyplex alone and yielded higher luciferase expression by providing protection against endosomal degradation. Overall, the monodispersed, tunable hollow spheres with a capability of post-functionalization can provide an exciting new opportunity for use in a range of therapeutic and diagnostic applications. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：382 / 392

页数：11

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