Thermoresponsive self-assembled elastin-based nanoparticles for delivery of BMPs

被引:134
作者
Bessa, Paulo C. [1 ,2 ,3 ,4 ]
Machado, Raul [1 ]
Nuernberger, Sylvia [4 ]
Dopler, Daniela [4 ]
Banerjee, Asmita [4 ]
Cunha, Antonio M. [7 ]
Carlos Rodriguez-Cabello, J. [5 ,6 ]
Redl, Heinz [4 ]
van Griensven, Martijn [4 ]
Reis, Rui L. [2 ,3 ]
Casal, Margarida [1 ]
机构
[1] Univ Minho, Dept Biol, CBMA Ctr Mol & Environm Biol, P-4710057 Braga, Portugal
[2] Univ Minho, Res Grp 3Bs, Headquarters European Inst Excellence Tissue Engn, P-4806909 Taipas, Guimaraes, Portugal
[3] PT Associated Lab, IBB, Guimaraes, Portugal
[4] AUVA Res Ctr, Ludwig Boltzmann Inst Expt & Clin Traumatol, A-1200 Vienna, Austria
[5] Univ Valladolid, ETSII, Dept Fis Mat Condensada, BIOFORGE Res Grp, E-47011 Valladolid, Spain
[6] CIBER BBN, Valladolid, Spain
[7] Univ Minho, Dept Polymer Engn, Inst Polymers & Composites, P-4800058 Guimaraes, Portugal
关键词
Elastin-like polymer; Thermoresponsive; Delivery; Nanoparticles; BMP-2; BMP-14; BONE MORPHOGENETIC PROTEINS; GROWTH-FACTOR; IN-VITRO; GROWTH/DIFFERENTIATION FACTOR-5; DIFFERENTIATION; BIOCOMPATIBILITY; PROLIFERATION; ANGIOGENESIS; BIOPOLYMERS; EXPRESSION;
D O I
10.1016/j.jconrel.2009.11.003
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Elastin-like polymers are a new type of protein-based polymers that display interesting properties in the biomaterial field. Bone morphogenetic proteins (BMPs) are cytokines with a strong ability to promote new bone formation. In this work, we explored the use of elastin-like nanoparticles (average size 237.5 +/- 3.0 nm), created by thermoresponsive self-assembly, for the combined release of bone morphogenetic protein-2 (BMP-2) and bone morphogenetic protein-14 (BMP-14). These BMPs could be encapsulated at high efficiency into the elastin-like particles and delivered in a sustained way for 14 days. The activity of the growth factors was retained, as shown by the induction of ALP activity and osteogenic mineralization in C2C12 cells. Increased bioactivity was observed with a combined release of BMP-2 and BMP-14. This approach shows a significant potential for future tissue engineering applications in bone. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码:312 / 318
页数:7
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