A Direct Technique for Magnetic Functionalization of Living Human Cells

被引：55

作者：

Dzamukova, Maria R. ^{[1
]}

Zamaleeva, Alsu I. ^{[1
]}

Ishmuchametova, Dilara G. ^{[1
]}

Osin, Yuri N. ^{[1
]}

Kiyasov, Andrey P. ^{[2
]}

Nurgaliev, Danis K. ^{[1
]}

Ilinskaya, Olga N. ^{[1
]}

Fakhrullin, Rawil F. ^{[1
]}

机构：

[1] Kazan Idel Buye Volga Reg Fed Univ, Biomat & Nanomat Grp, Fac Biol & Soil, Kazan 420008, Republic Of Tat, Russia

[2] Kazan State Med Univ, Dept Normal Anat, Republic Of Tatarstan 420012, Russia

来源：

LANGMUIR | 2011年 / 27卷 / 23期

关键词：

POLYELECTROLYTE SHELLS; TUMOR-CELLS; NANOPARTICLES; YEAST;

D O I：

10.1021/la203839v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Functionalized living cells are regarded as effective tools in directed cell delivery and tissue engineering. Here we report the facile functionalization of viable isolated HeLa cells with superparamagnetic cationic nanoparticles via a single-step biocompatible process. Nanoparticles are localized on the cellular membranes and do not penetrate into the cytoplasm. The magnetically responsive cells are viable and able to colonize and grow on substrates. Magnetically facilitated microorganization of functionalized cells into viable living clusters is demonstrated. We believe that the technique described here may find a number of potential applications in cell-based therapies and in development of whole-cell biosensors.

引用

收藏

页码：14386 / 14393

页数：8

相关论文

共 31 条

[1] Electrostatic layer-by-layer nanoassembly on biological microtemplates: Platelets [J].

Ai, H ;

Fang, M ;

Jones, SA ;

Lvov, YM .

BIOMACROMOLECULES, 2002, 3 (03) :560-564

[2] Encapsulation of Bacterial Spores in Nanoorganized Polyelectrolyte Shells [J].

Balkundi, Shantanu S. ;

Veerabadran, Nalinkanth G. ;

Eby, D. Matthew ;

Johnson, Glenn R. ;

Lvov, Yuri M. .

LANGMUIR, 2009, 25 (24) :14011-14016

[3] CHARACTERIZATION OF UPTAKE AND HYDROLYSIS OF FLUORESCEIN DIACETATE AND CARBOXYFLUORESCEIN DIACETATE BY INTRACELLULAR ESTERASES IN SACCHAROMYCES-CEREVISIAE, WHICH RESULT IN ACCUMULATION OF FLUORESCENT PRODUCT [J].

BREEUWER, P ;

DROCOURT, JL ;

BUNSCHOTEN, N ;

ZWIETERING, MH ;

ROMBOUTS, FM ;

ABEE, T .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) :1614-1619

[4] EGFR-Targeted Magnetic Nanoparticle Heaters Kill Cancer Cells without a Perceptible Temperature Rise [J].

Creixell, Mar ;

Bohorquez, Ana C. ;

Torres-Lugo, Madeline ;

Rinaldi, Carlos .

ACS NANO, 2011, 5 (09) :7124-7129

[5] Preparation of epidermal growth factor (EGF) conjugated iron oxide nanoparticles and their internalization into colon cancer cells [J].

Creixell, Mar ;

Herrera, Adriana P. ;

Ayala, Vanessa ;

Latorre-Esteves, Magda ;

Perez-Torres, Marianela ;

Torres-Lugo, Madeline ;

Rinaldi, Carlos .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2010, 322 (15) :2244-2250

[6] Single living cell encapsulation in nano-organized polyelectrolyte shells [J].

Diaspro, A ;

Silvano, D ;

Krol, S ;

Cavalleri, O ;

Gliozzi, A .

LANGMUIR, 2002, 18 (13) :5047-5050

[7] Quartz crystal microbalance immunosensor for the detection of antibodies to double-stranded DNA [J].

Fakhrullin, Rawil F. ;

Vinter, Victor G. ;

Zamaleeva, Alsu I. ;

Matveeva, Maria V. ;

Kourbanov, Roustem A. ;

Temesgen, Belayhun K. ;

Ishmuchametova, Dilara G. ;

Abramova, Zinaida I. ;

Konovalova, Olga A. ;

Salakhov, Myakzyum K. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (02) :367-375

[8] Interfacing Living Unicellular Algae Cells with Biocompatible Polyelectrolyte-Stabilised Magnetic Nanoparticles [J].

Fakhrullin, Rawil F. ;

Shlykova, Lubov V. ;

Zamaleeva, Alsu I. ;

Nurgaliev, Danis K. ;

Osin, Yuri N. ;

Garcia-Alonso, Javier ;

Paunov, Vesselin N. .

MACROMOLECULAR BIOSCIENCE, 2010, 10 (10) :1257-1264

[9] A direct technique for preparation of magnetically functionalised living yeast cells [J].

Fakhrullin, Rawil F. ;

Garcia-Alonso, Javier ;

Paunov, Vesselin N. .

SOFT MATTER, 2010, 6 (02) :391-397

[10] Microscreening toxicity system based on living magnetic yeast and gradient chips [J].

Garcia-Alonso, Javier ;

Fakhrullin, Rawil F. ;

Paunov, Vesselin N. ;

Shen, Zheng ;

Hardege, Joerg D. ;

Pamme, Nicole ;

Haswell, Stephen J. ;

Greenway, Gillian M. .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2011, 400 (04) :1009-1013

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