Covalent Cell Surface Functionalization of Human Fetal Osteoblasts for Tissue Engineering

被引：17

作者：

Borcard, Francoise ^{[1
]}

Godinat, Aurelien ^{[1
]}

Staedler, Davide ^{[1
]}

Blanco, Horacio Comas ^{[1
]}

Dumont, Anne-Laure ^{[1
]}

Chapuis-Bernasconi, Catherine ^{[2
]}

Scaletta, Corinne ^{[3
]}

Applegate, Lee Ann ^{[3
]}

Juillerat, Franziska Krauss ^{[4
]}

Gonzenbach, Urs T.

Gerber-Lemaire, Sandrine ^{[1
]}

Juillerat-Jeanneret, Lucienne ^{[2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn, CH-1015 Lausanne, Switzerland

[2] Univ Inst Pathol, CH-1011 Lausanne, Switzerland

[3] CHUV UNIL, Cellular Therapy Unit, Dept Musculoskeletal Med, CH-1011 Lausanne, Switzerland

[4] ETHZ, Nonmetall Inorgan Mat, CH-8093 Zurich, Switzerland

来源：

BIOCONJUGATE CHEMISTRY | 2011年 / 22卷 / 07期

基金：

瑞士国家科学基金会;

关键词：

NEWLY SYNTHESIZED PROTEINS; CLICK CHEMISTRY; COPPER-FREE; LIVING CELLS; SELECTIVITY; AZIDE;

D O I：

10.1021/bc200147m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne derivatives with cellular azido-expressing proteins. The incorporation via a tetraethylene glycol linker of a dipeptide and a reporter biotin allowed the proof of concept for the introduction of cell-specific peptide ligands and allowed us to follow the reaction in living cells. Tuning the conditions of the click reaction resulted in chemical functionalization of living human fetal osteoblasts with excellent cell survival.

引用

页码：1422 / 1432

页数：11

共 26 条

[1] The role of ECM proteins and protein fragments in guiding cell behavior in regenerative medicine [J].

Barker, Thomas H. .

BIOMATERIALS, 2011, 32 (18) :4211-4214

[2] Bioorthogonal click chemistry: Covalent labeling in living systems [J].

Baskin, Jeremy M. ;

Bertozzi, Carolyn R. .

QSAR & COMBINATORIAL SCIENCE, 2007, 26 (11-12) :1211-1219

[3] Advantages of RGD peptides for directing cell association with biomaterials [J].

Bellis, Susan L. .

BIOMATERIALS, 2011, 32 (18) :4205-4210

[4] Click Chemistry and Bioorthogonal Reactions: Unprecedented Selectivity in the Labeling of Biological Molecules [J].

Best, Michael D. .

BIOCHEMISTRY, 2009, 48 (28) :6571-6584

[5] Second-generation difluorinated cyclooctynes for copper-free click chemistry [J].

Codelli, Julian A. ;

Baskin, Jeremy M. ;

Agard, Nicholas J. ;

Berozzi, Carolyn R. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (34) :11486-11493

[6] Azide: A Unique Dipole for Metal-Free Bioorthogonal Ligations [J].

Debets, Marjoke F. ;

van der Doelen, Christianus W. J. ;

Rutjes, Floris P. J. T. ;

van Delft, Floris L. .

CHEMBIOCHEM, 2010, 11 (09) :1168-1184

[7] Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT) [J].

Dieterich, Daniela C. ;

Link, A. James ;

Graumann, Johannes ;

Tirrell, David A. ;

Schuman, Erin M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (25) :9482-9487

[8] In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons [J].

Dieterich, Daniela C. ;

Hodas, Jennifer J. L. ;

Gouzer, Geraldine ;

Shadrin, Ilya Y. ;

Ngo, John T. ;

Triller, Antoine ;

Tirrell, David A. ;

Schuman, Erin M. .

NATURE NEUROSCIENCE, 2010, 13 (07) :897-U149

[9] RECENT ADVANCES IN THE STAUDINGER REACTION [J].

GOLOLOBOV, YG ;

KASUKHIN, LF .

TETRAHEDRON, 1992, 48 (08) :1353-1406

[10]

Griffin R J, 1994, Prog Med Chem, V31, P121, DOI 10.1016/S0079-6468(08)70020-1

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