Crystal structure of the dimeric unswapped form of bovine seminal ribonuclease

被引：24

作者：

Berisio, R

Sica, F

De Lorenzo, C

Di Fiore, A

Piccoli, R

Zagari, A

Mazzarella, L

机构：

[1] CNR, Ist Biostruct & Bioimmagini, I-80134 Naples, Italy

[2] Univ Naples Federico II, Dipartimento Chim, I-80126 Naples, Italy

[3] Univ Naples Federico II, Dept Chim Biol, I-80126 Naples, Italy

[4] Univ Naples Federico II, Fac Sci Biotecnol, Naples, Italy

来源：

FEBS LETTERS | 2003年 / 554卷 / 1-2期

关键词：

three-dimensional domain swapping; conformational dimorphism; crystal structure; ribonuclease;

D O I：

10.1016/S0014-5793(03)01114-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine seminal ribonuclease is a unique case of protein dimorphism, since it exists in two dimeric forms, with different biological and kinetic behavior, which interconvert into one another through three-dimensional swapping. Here we report the crystal structure, at 2.2 Angstrom resolution, of the unswapped form of bovine seminal ribonuclease. Besides completing the structural definition of bovine seminal ribonuclease conformational dimorphism, this study provides the structural basis to explain the dependence of the enzyme cooperative effects on its swapping state. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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页码：105 / 110

页数：6

相关论文

共 39 条

[1] BS-RNase tetramers: An example of domain-swapped oligomers [J].

Adinolfi, S ;

Piccoli, R ;

Sica, F ;

Mazzarella, L .

FEBS LETTERS, 1996, 398 (2-3) :326-332

[2] The swapping of terminal arms in ribonucleases: Comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases [J].

Avitabile, F ;

Alfano, C ;

Spadaccini, R ;

Crescenzi, O ;

D'Ursi, AM ;

D'Alessio, G ;

Tancredi, T ;

Picone, D .

BIOCHEMISTRY, 2003, 42 (29) :8704-8711

[3] 3D DOMAIN SWAPPING - A MECHANISM FOR OLIGOMER ASSEMBLY [J].

BENNETT, MJ ;

SCHLUNEGGER, MP ;

EISENBERG, D .

PROTEIN SCIENCE, 1995, 4 (12) :2455-2468

[4] Protein titration in the crystal state [J].

Berisio, R ;

Lamzin, VS ;

Sica, F ;

Wilson, KS ;

Zagari, A ;

Mazzarella, L .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 292 (04) :845-854

[5] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[6] THE ANTITUMOR ACTION OF SEMINAL RIBONUCLEASE AND ITS QUATERNARY CONFORMATIONS [J].

CAFARO, V ;

DELORENZO, C ;

PICCOLI, R ;

BRACALE, A ;

MASTRONICOLA, MR ;

DIDONATO, A ;

DALESSIO, G .

FEBS LETTERS, 1995, 359 (01) :31-34

[7] The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins toward oligomerization [J].

Canals, A ;

Pous, J ;

Guasch, A ;

Benito, A ;

Ribó, M ;

Vilanova, M ;

Coll, M .

STRUCTURE, 2001, 9 (10) :967-976

[8] Origin of dimeric structure in the ribonuclease superfamily [J].

Ciglic, MI ;

Jackson, PJ ;

Raillard, SA ;

Haugg, M ;

Jermann, TM ;

Opitz, JG ;

Trabesinger-Rüf, N ;

Benner, SA .

BIOCHEMISTRY, 1998, 37 (12) :4008-4022

[9] The evolutionary transition from monomeric to oligomeric proteins: tools, the environment, hypotheses [J].

D'Alessio, G .

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1999, 72 (03) :271-298

[10]

D'Alessio G, 1997, RIBONUCLEASES STRUCT, P383

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