Mapping the Core of the Arabidopsis Circadian Clock Defines the Network Structure of the Oscillator

被引：401

作者：

Huang, W. ^{[1
]}

Perez-Garcia, P. ^{[1
]}

Pokhilko, A. ^{[2
]}

Millar, A. J. ^{[2
,3
]}

Antoshechkin, I. ^{[4
,5
]}

Riechmann, J. L. ^{[1
,4
,5
]}

Mas, P. ^{[1
]}

机构：

[1] Ctr Res Agr Genom, Barcelona 08193, Spain

[2] Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3JH, Midlothian, Scotland

[3] Ctr Syst Biol Edinburgh, Edinburgh EH9 3JD, Midlothian, Scotland

[4] CALTECH, Div Biol, Pasadena, CA 91125 USA

[5] Inst Catalana Rec & Estudis Avancats, Barcelona 08010, Spain

来源：

SCIENCE | 2012年 / 336卷 / 6077期

基金：

英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

PSEUDO-RESPONSE REGULATORS; TOC1; RHYTHMS; TIME; EXPRESSION; THALIANA; GIGANTEA; GENE;

D O I：

10.1126/science.1219075

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In many organisms, the circadian clock is composed of functionally coupled morning and evening oscillators. In Arabidopsis, oscillator coupling relies on a core loop in which the evening oscillator component TIMING OF CAB EXPRESSION 1 (TOC1) was proposed to activate a subset of morning-expressed oscillator genes. Here, we show that TOC1 does not function as an activator but rather as a general repressor of oscillator gene expression. Repression occurs through TOC1 rhythmic association to the promoters of the oscillator genes. Hormone-dependent induction of TOC1 and analysis of RNA interference plants show that TOC1 prevents the activation of morning-expressed genes at night. Our study overturns the prevailing model of the Arabidopsis circadian clock, showing that the morning and evening oscillator loops are connected through the repressing activity of TOC1.

引用

页码：75 / 79

页数：5

共 27 条

[1] Reciprocal regulation between TOC1 and LHY/CCA1 within the Arabidopsis circadian clock [J].