Mechanism of estrogens-induced increases in intracellular Ca2+ in PC3 human prostate cancer cells

被引:30
作者
Huang, JK [1 ]
Jan, CR
机构
[1] Kaohsiung Vet Gen Hosp, Dept Surg, Div Urol, Kaohsiung 813, Taiwan
[2] Natl Yang Ming Univ, Sch Med, Taipei 112, Taiwan
[3] Kaohsiung Vet Gen Hosp, Dept Med Educ & Res, Kaohsiung 813, Taiwan
[4] Natl Sun Yat Sen Univ, Dept Biol, Kaohsiung 80424, Taiwan
[5] Natl Sun Yat Sen Univ, Inst Life Sci, Kaohsiung 80424, Taiwan
关键词
17; beta-estradiol; Ca2+; diethylstilbestrol; PC3; prostate cancer cells;
D O I
10.1002/pros.1057
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
BACKGROUND. The effect of estrogens (diethylstilbestrol [DES], 17 beta -estradiol) on intracellular Ca2+ concentrations ([Ca2+](i)) in hormone-insensitive PC3 human prostate cancer cells was examined. METHODS. [Ca2+](i) changes in suspended cells were measured by using the Ca2+-sensitive fluorescent dye fura-2. RESULTS. Estrogens (1-20 muM) increased [Ca2+](i) concentration-dependently with DES being more potent. Ca2+ removal inhibited 50+/-10% of the signal. In Ca(2+-)free medium, pretreatment with 20 muM estrogens abolished the [Ca2+](i) increases induced by 2 muM carbonylcyanide m-cholorophenylhydrazone (CCCP, a mitochondrial uncoupler) and 1 muM thapsigargin (an endoplasmic reticulum Ca2+ pum inhibitor), but pretreatment with CCCP and thapsigargin did not alter DES-induced Ca2+ increased [Ca2+](i) in cells pretreated with 1-20 muM estrogens in Ca(2+-)free medium. Pretreatment with 1 muM U73122 to block phospholipase C-coupled inositol 1,4,5-triphosphate formation did not alter estrogens-induced Ca2+ release. The effect of 20 muM estrogen on [Ca2+](i) was not affected by pretreatment with 0.1 muM estrogens. CONCLUSIONS. Estrogen induced significant Ca2+ release and Ca2+ influx in an inositol 1,4,5-triphosphate-independent manner in PC3 cells. These effects of estrogens on Ca2+ signaling appear to be nongenomic. Prostate 47:141-148, 2001. (C) 2001 Wiley-Liss, Inc.
引用
收藏
页码:141 / 148
页数:8
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