Osteoblast recruitment from stem cells does not decrease by age at late adulthood

被引：103

作者：

Leskelä, HV

Risteli, J

Niskanen, S

Koivunen, J

Ivaska, KK

Lehenkari, P

机构：

[1] Univ Oulu, Dept Anat & Cell Biol, FIN-90014 Oulu, Finland

[2] Univ Turku, Inst Biomed, Dept Anat, FIN-20520 Turku, Finland

[3] Univ Oulu, Dept Surg, FIN-90014 Oulu, Finland

[4] Univ Kuopio, Dept Clin Chem, FIN-70211 Kuopio, Finland

[5] Univ Oulu, Dept Clin Chem, FIN-90014 Oulu, Finland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 311卷 / 04期

关键词：

mesenchymal stem cells; bone marrow; differentiation; osteoblasts; aging; PINP; ALP; matrix mineralization;

D O I：

10.1016/j.bbrc.2003.10.095

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was aimed to characterize the ability of human bone marrow mesenchymal stem cells (MSC) to differentiate into osteoblasts in vitro. Twenty-three women and 20 men at late adulthood (52-92 years of age) were selected for the study. MSCs were isolated and cultured in vitro and alkaline phosphatase (ALP) activity, secretion of amino-terminal propeptide of type I procollagen (PINP), type III procollagen (PIIINP) and osteocalcin were analyzed. Matrix mineralization was analyzed by the von Kossa staining and by calcium quantification. We found that the ALP and PINP levels compared with control increased to 2.8- and 2.9-fold, respectively, when cells were cultured for three weeks. ALP activity, PINP and calcium deposition in response to dexamethasone treatment increased by age in women and was unchanged in men. Overall our data suggests that the osteogenic potential of MSCs does not decrease by age in either women or men at late adulthood. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：1008 / 1013

页数：6

共 29 条

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