Synthesis, characterization, and in vitro biological evaluation of highly stable diversely functionalized superparamagnetic iron oxide nanoparticles

被引:36
作者
Bhattacharya, Dipsikha [1 ]
Sahu, Sumanta K. [1 ]
Banerjee, Indranil [2 ]
Das, Manasmita [1 ]
Mishra, Debashish [2 ]
Maiti, Tapas K. [2 ]
Pramanik, Panchanan [1 ]
机构
[1] Indian Inst Technol, Dept Chem, Kharagpur 721302, W Bengal, India
[2] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
关键词
SPIONs; Chitosan; Cancer; MRI; Nanoconjugates; Colloids; Nanomedicine; MAGNETIC NANOPARTICLES; CHITOSAN; STABILITY; ADSORPTION; SIZE;
D O I
10.1007/s11051-011-0362-7
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this article, we report the design and synthesis of a series of well-dispersed superparamagnetic iron oxide nanoparticles (SPIONs) using chitosan as a surface modifying agent to develop a potential T (2) contrast probe for magnetic resonance imaging (MRI). The amine, carboxyl, hydroxyl, and thiol functionalities were introduced on chitosan-coated magnetic probe via simple reactions with small reactive organic molecules to afford a series of biofunctionalized nanoparticles. Physico-chemical characterizations of these functionalized nanoparticles were performed by TEM, XRD, DLS, FTIR, and VSM. The colloidal stability of these functionalized iron oxide nanoparticles was investigated in presence of phosphate buffer saline, high salt concentrations and different cell media for 1 week. MRI analysis of human cervical carcinoma (HeLa) cell lines treated with nanoparticles elucidated that the amine-functionalized nanoparticles exhibited higher amount of signal darkening and lower T (2) relaxation in comparison to the others. The cellular internalization efficacy of these functionalized SPIONs was also investigated with HeLa cancer cell line by magnetically activated cell sorting (MACS) and fluorescence microscopy and results established selectively higher internalization efficacy of amine-functionalized nanoparticles to cancer cells. These positive attributes demonstrated that these nanoconjugates can be used as a promising platform for further in vitro and in vivo biological evaluations.
引用
收藏
页码:4173 / 4188
页数:16
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