DLP printed β-tricalcium phosphate functionalized ceramic scaffolds promoted angiogenesis and osteogenesis in long bone defects

被引：25

作者：

Bi, Gangyuan ^{[2
,3
,4
,5
,6
]}

Mo, Lina ^{[1
,3
,4
,5
,6
]}

Liu, Sa ^{[1
,3
,4
,5
,6
]}

Zhong, Xiupeng ^{[1
,3
,4
,5
,6
]}

Yang, Junzhong ^{[1
,3
,4
,5
,6
]}

Yuan, Zhongrun ^{[1
,3
,4
,5
,6
]}

Chen, Shenggui ^{[7
]}

Ren, Li ^{[1
,3
,4
,5
,6
]}

机构：

[1] South China Univ Technol, Sch Mat Sci & Engn, Guangzhou 510641, Peoples R China

[2] South China Univ Technol, Sch Biomed Sci & Engn, Guangzhou 511442, Peoples R China

[3] South China Univ Technol, Natl Engn Res Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Peoples R China

[4] South China Univ Technol, Key Lab Biomed Engn Guangdong Prov, Guangzhou 510006, Peoples R China

[5] South China Univ Technol, Minist Educ, Key Lab Biomed Mat & Engn, Guangzhou 510006, Peoples R China

[6] South China Univ Technol, Innovat Ctr Tissue Restorat & Reconstruct, Guangzhou 510006, Peoples R China

[7] Dongguan Univ Technol, Sch Mech Engn, Dongguan 523808, Peoples R China

来源：

CERAMICS INTERNATIONAL | 2022年 / 48卷 / 18期

关键词：

Long bone defect; Functionalized scaffold; Angiogenesis; Osteogenesis; Digital light processing; SUBSTITUTES;

D O I：

10.1016/j.ceramint.2022.05.310

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

081705 [工业催化]; 082905 [生物质能源与材料];

摘要：

Nowadays, the repair of long bone defects remains a clinical challenge mainly due to poor oxygen and nutrients delivery. In this study, beta-tricalcium phosphate (beta-TCP) porous ceramic scaffolds were prepared by digital light processing (DLP) and gradient sintering process. The functionalization of scaffolds was achieved by loading hyaluronic acid-dopamine (HA-DA) coating or sphingosine 1-phosphate/hyaluronic acid-dopamine (S1P/HADA) coating, which solved the problem of oxygen and nutrients delivery to a certain extent by promoting blood vessels growth. Cytocompatibility assay, qRT-PCR, Alkaline phosphatase (ALP) staining and quantitative analysis demonstrated that the S1P/HA-DA/TCP scaffolds significantly promoted the proliferation and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (mBMSCs). Long bone defects (22 mm), rarely reported in previous studies, were constructed on the radius of rabbits. Animal experiments showed excellent early angiogenesis and bone repair in HA-DA/TCP and S1P/HA-DA/TCP groups. In particular, the S1P/HA-DA/TCP scaffolds enhanced bone regeneration and osseointegration. Overall, these functionalized scaffolds had an effective repair on long bone defects that would have great potential for clinical applications.

引用

页码：26274 / 26286

页数：13

共 47 条

[1]

β-TCP granules mixed with reticulated hyaluronic acid induce an increase in bone apposition [J].