Development of a chemically defined medium for the growth of Leuconostoc mesenteroides

被引:32
作者
Foucaud, C [1 ]
Francois, A [1 ]
Richard, J [1 ]
机构
[1] INRA, UNITE RECH LAITIERES, F-78350 JOUY EN JOSAS, FRANCE
关键词
D O I
10.1128/AEM.63.1.301-304.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要
A chemically defined medium for the growth of Leuconostoc mesenteroides was developed. This medium contained lactose, Mn2+, Mg2+, 12 amino acids, eight vitamins, adenine, uracil, and Tween 80. We showed the beneficial effect of aerobic conditions on growth and that potassium phosphate (135 mM) is a suitable buffer. The growth rate in this medium was 0.85 +/- 0.10 h(-1) for the six strains examined, and cell densities up to 3.5 x 10(9) CFU/ml were attained.
引用
收藏
页码:301 / 304
页数:4
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