Mapping of the binding site for Mannheimia haemolytica leukotoxin within bovine CD18

被引：14

作者：

Dileepan, T ^{[1
]}

Kannan, MS ^{[1
]}

Walcheck, B ^{[1
]}

Thumbikat, P ^{[1
]}

Maheswaran, SK ^{[1
]}

机构：

[1] Univ Minnesota, Dept Vet & Biomed Sci, St Paul, MN 55108 USA

来源：

INFECTION AND IMMUNITY | 2005年 / 73卷 / 08期

关键词：

D O I：

10.1128/IAI.73.8.5233-5237.2005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

To map the site involved in Mannheimia haemolytica leukotoxin (LktA) binding and biological activity within bovine CD18, bovine X human CD18 chimeric constructs were generated and coexpressed with bovine CD11a in K562 cells. Studies with the chimeric leukocyte function-associated antigen 1 transductants demonstrate that the site required for LktA binding and biological effects resides within amino acid residues 500 and 600 of the extracellular region of bovine CD18.

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收藏

页码：5233 / 5237

页数：5

相关论文

共 20 条

[1]

Response of the ruminant respiratory tract to Mannheimia (Pasteurella) haemolytica [J].

Ackermann, MR ;

Brogden, KA .

MICROBES AND INFECTION, 2000, 2 (09) :1079-1088

[2]

Bovine CD18 is necessary and sufficient to mediate Mannheimia (Pasteurella) haemolytica leukotoxin-induced cytolysis [J].

Deshpande, MS ;

Ambagala, TC ;

Ambagala, APN ;

Kehrli, ME ;

Srikumaran, S .

INFECTION AND IMMUNITY, 2002, 70 (09) :5058-5064

[3]

PCR-MEDIATED RECOMBINATION AND MUTAGENESIS [J].

HORTON, RM .

MOLECULAR BIOTECHNOLOGY, 1995, 3 (02) :93-99

[4]

Pasteurella haemolytica leukotoxin and endotoxin induced cytokine gene expression in bovine alveolar macrophages requires NF-κB activation and calcium elevation [J].

Hsuan, SL ;

Kannan, MS ;

Jeyaseelan, S ;

Prakash, YS ;

Malazdrewich, C ;

Abrahamsen, MS ;

Sieck, GC ;

Maheswaran, SK .

MICROBIAL PATHOGENESIS, 1999, 26 (05) :263-273

[5]

Folding of the conserved domain but not of flanking regions in the integrin beta(2) subunit requires association with the alpha subunit [J].

Huang, CC ;

Lu, CF ;

Springer, TA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (07) :3156-3161

[6]

Lymphocyte function-associated antigen 1 is a receptor for Pasteurella haemolytica leukotoxin in bovine leukocytes [J].

Jeyaseelan, S ;

Hsuan, SL ;

Kannan, MS ;

Walcheck, B ;

Wang, JF ;

Kehrli, ME ;

Lally, ET ;

Sieck, GC ;

Maheswaran, SK .

INFECTION AND IMMUNITY, 2000, 68 (01) :72-79

[7]

Jeyaseelan Samithamby, 2002, Animal Health Research Reviews, V3, P69, DOI 10.1079/AHRR200242

[8]

A missense mutation in the β-2 integrin gene (ITGB2) causes canine leukocyte adhesion deficiency [J].

Kijas, JMH ;

Bauer, TR ;

Gäfvert, S ;

Marklund, S ;

Trowald-Wigh, G ;

Johannisson, A ;

Hedhammar, Å ;

Binns, M ;

Juneja, RK ;

Hickstein, DD ;

Andersson, L .

GENOMICS, 1999, 61 (01) :101-107

[9]

RTX toxins recognize a beta 2 integrin on the surface of human target cells [J].

Lally, ET ;

Kieba, IR ;

Sato, A ;

Green, CL ;

Rosenbloom, J ;

Korostoff, J ;

Wang, JF ;

Shenker, BJ ;

Ortlepp, S ;

Robinson, MK ;

Billings, PC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (48) :30463-30469

[10]

Bovine CD18 identified as a species specific receptor for Pasteurella haemolytica leukotoxin [J].

Li, J ;

Clinkenbeard, KD ;

Ritchey, JW .

VETERINARY MICROBIOLOGY, 1999, 67 (02) :91-97