Adsorption of bovin serum albumin (BSA) onto the magnetic chitosan nanoparticles prepared by a microemulsion system

被引:92
作者
Wang, Yujun [1 ]
Wang, Xianghua [1 ]
Luo, Guangsheng [1 ]
Dai, Youyuan [1 ]
机构
[1] Tsinghua Univ, Dept Chem Engn, State Key Lab Chem Engn, Beijing 100084, Peoples R China
基金
中国国家自然科学基金;
关键词
magnetic chitosan nanoparticles; bovine serum albumin (BSA); adsorption;
D O I
10.1016/j.biortech.2007.08.017
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The adsorption characteristics of BSA onto the magnetic chitosan nanoparticles have been investigated in this paper. The magnetic chitosan nanoparticles were prepared by adding the basic precipitant of NaOH solution into a W/O microemulsion system. The morphology of magnetic chitosan nanoparticles was observed by transmission electron microscope (TEM). It was found that the diameter of magnetic chitosan nanoparticles was from 10 nm to 20 nm, and the nanoparticles suspending in the aqueous solution could easily aggregate by a magnet, which suggested that the nanoparticles had good magnetic characteristics. The BSA adsorption experiment indicated that when pH of BSA solution was equal to 4, the maximum adsorption loading reached 110 mg/g. Through measuring the zeta potential of BSA solution and the magnetic nanoparticles, it was found that under this situation the surface of BSA took the negative charge, but the magnetic nanoparticles took the positive charge. Due to the small diameter, the adsorption equilibrium of BSA onto the nanoparticles reached very quickly within 10 min. The adsorption equilibrium of BSA onto the magnetic chitosan nanoparticles fitted well with the Freundlich model. The experimental results showed that the magnetic chitosan nanoparticles have potential to be used for the quick pretreatment in the protein analysis process. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3881 / 3884
页数:4
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