High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology

被引：29

作者：

Burgemeister, R ^{[1
]}

Gangnus, R ^{[1
]}

Haar, B ^{[1
]}

Schütze, K ^{[1
]}

Sauer, U ^{[1
]}

机构：

[1] PALM Microlaser Technol AG, D-82347 Bernried, Germany

来源：

PATHOLOGY RESEARCH AND PRACTICE | 2003年 / 199卷 / 06期

关键词：

LMPC; laser microdissection; gene expression; RNA integrity; homogeneous material;

D O I：

10.1078/0344-0338-00442

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Isolation of intact RNA in high quality is the first and often the most critical step in performing many fundamental molecular biology experiments, and is essential for many techniques used in gene expression analysis. As many factors influence nucleic acid preservation, RNA isolation should include some important steps before and after the actual RNA extraction. We tested the influence of fixation and staining protocols regarding RNA integrity and concentration. A factor that is often underestimated is the absolute necessity for homogenous starting materials. Application of the LMPC technology allows for a rapid and highly precise procurement of purified cell populations suitable for a variety of downstream analyses.

引用

页码：431 / 436

页数：6