The three-dimensional structure of the analgesic α-conotoxin, RgIA

被引：32

作者：

Clark, Richard J. ^{[1
]}

Daly, Norelle L. ^{[1
]}

Halai, Reena ^{[1
]}

Nevin, Simon T. ^{[2
]}

Adams, David J. ^{[2
]}

Craik, David J. ^{[1
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Sch Biomed Sci, Brisbane, Qld 4072, Australia

来源：

FEBS LETTERS | 2008年 / 582卷 / 05期

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

conotoxin; nuclear magnetic resonance; analgesic; oxidative folding; disulfide isomers;

D O I：

10.1016/j.febslet.2008.01.027

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The alpha-conotoxin RgIA is a selective antagonist of the alpha 9 alpha 10 nicotinic acetylcholine receptor and has been shown to be a potent analgesic and reduces nerve injury associated inflammation. RgIA was chemically synthesized and found to fold into two disulfide isomers, globular and ribbon. The native globular isomer inhibited ACh-evoked currents reversibly in oocytes expressing rat alpha 9 alpha 10 nAChRs but the ribbon isomer was inactive. We determined the three-dimensional structure of RgIA using NMR methods to assist in elucidating the molecular role of RgIA in analgesia and inflammation. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：597 / 602

页数：6

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